Introduction to Adenovirus Specific for Suspension Cells The adenoviral vectors provided by Genemedi are based on human adenovirus type 5 (Ad5), which is replication-incompetent (-E1/-E3) and can’t be integrated into host genome, guaranteeing the security for subsequent operations. Adv packaging by Genemedi shows almost 100% gene delivery in most cell types in the recombinant protein […]
Category Archives: gene therapy
Adenovirus vector -Introduction Landscape, protocol and guidelines of adenovirus vector system (adeasy and admax), adenovirus production, adenovirus mediated gene transduction in vitro and in vivo are described below. The detail information is mentioned about adenoviral vector (adenovirus plasmids) cloning, adenovirus packaging, purification and the insight of adenovirus gene delivery and gene therapy) Adenovirus vector -Index […]
Selected Publications using Genemed’s Autophagy Products/Services Publications Link to the article Products/Services T. Yu et.al, ‘Fusobacterium Nucleatum Promotes Chemoresistance to Colorectal Cancer by Modulating Autophagy’, Cell, 170 (2017), 548-63 e16. http://www.ncbi.nlm.nih.gov/pubmed/28753429 Autophagy flux RFP-GFP-LC3 biosensor M. Liu et.al, ‘Sirt6 Deficiency Exacerbates Podocyte Injury and Proteinuria through Targeting Notch Signaling’, Nat Commun, 8 (2017), 413. http://www.ncbi.nlm.nih.gov/pubmed/28871079 […]
Selected Publications using Genemed’s LipoGene™ Transfection Reagent Publications Link to the article Products/Services X. Ma et.al, ‘Hippo Signaling Promotes Jnk-Dependent Cell Migration’, Proc Natl Acad Sci U S A, 114 (2017), 1934-39. http://www.ncbi.nlm.nih.gov/pubmed/28174264 LipoGene™ Transfection Reagent Y. Li et.al, ‘A Novel Epigenetic Aml1-Eto/Thap10/Mir-383 Mini-Circuitry Contributes to T(8;21) Leukaemogenesis’, EMBO Mol Med, 9 (2017), 933-49. http://www.ncbi.nlm.nih.gov/pubmed/28539478 […]
Introduction-LC3 Autophagy Biosensors Autophagy is a highly regulated homeostatic degradative process where cells destroy their own components via the lysosomal machinery and recycle them. This process plays both protective and deleterious roles in many diseases, including Alzheimer’s disease, aging, cancer, infection and Crohn’s disease. Elucidating the correlation between autophagy and apoptotic cell death has become […]
Q1. Are cell density (% confluence) and passage number important considerations for transfection? A: Yes, cell density is an important parameter in influencing transfection efficiency. If the seeding density is too low, some cytotoxicity may be observed. If the cell density is high, lower than expected transfection efficiency may be observed. Both issues may be […]
1. Why does adenovirus have a relatively higher immunogenecity compared with rAAV? The adenovirus without E1/E3 can express all of the other genes in the viral backbone and hence induces immunogenic responses, while rAAV does not have any of the AAV genes, thus no immunogenicity from viral protein. 2. What is the role of the […]
How can I choose the optimal AAV serotypes for my in vivo study? Genemedi has launched a comprehensive AAV production service. More than 12 AAV serotypes and a variety of capsid engineered AAV vectors are available for targeting different tissues and organs. Different AAV serotypes have different tissue tropism in vivo, the common serotypes and […]
Selected Publications using Genemed’s AAV Products/Services Publications Link to the article Products/Services H. Zhu, et. al, ‘Moderate Uv Exposure Enhances Learning and Memory by Promoting a Novel Glutamate Biosynthetic Pathway in the Brain’, Cell, 173 (2018), 1716-27 e17 http://www.ncbi.nlm.nih.gov/pubmed/29779945 AAV virus H. Yang et. al, ‘Laterodorsal Tegmentum Interneuron Subtypes Oppositely Regulate Olfactory Cue-Induced Innate Fear’, […]
CRISPR/Cas9 protocol Download Gene editing, also called genome editing, is a group of technologies to change the sequence of DNA in the genome. Several approaches to genome editing have been developed, including Zinc Finger, TALEN, and CRISPR/Cas9. Compared with the other gene editing tools, CRISPR/Cas9 system is faster, cheaper, more accurate, and more efficient, showing […]
