Infectious diseases continue to threaten the sustainability, productivity and growth of the poultry industry worldwide and some present a risk to public health. Many are also present in wild bird populations, with the potential to spill over into domestic birds. The avian infectious disease cause huge economic loss in poultry production and are of great significance in public health. However, they are usually not covered in the systems for reporting of animal diseases. Several methods have been employed to develop conventional assays to detect avian disease, Genemedi developed the antigen and antibody to detect the upper respiratory tract infection, newcastle disease, duck hepatitis, derzsy’s disease, infectious bursal disease, chlamydiosis, respiratory disease, arthritis, duck plague, avian influenza, cholecystitis, bacteremia, cholangitis, urinary tract infection (UTI), and traveler’s diarrhea, respiratory disease, avian metapneumovirus infection, treponeme, encephalomyelitis, infectious bronchitis, mycoplasmosis, nephritis, blackhead disease (histomoniasis), bordetellosis, campylobacteriosis, infectious anemia, chlamydia, duck septicaemia, Goose ‘flu, riemerellosis, polyserositis, egg drop syndrome, erysipeloid, fowl cholera, fowl typhoid, pullorum, fowlpox, gastroenteritis, hemorrhagic nephritis, enteritis of geese, hepatitis-splenomegaly syndrome, immunosuppression, inclusion body hepatitis, infectious bronchitis, infectious coryza, infectious laryngotracheitis, infectious synovitis, avian mycoplasmosis, infectious sinusitis, mycoplasma arthritis, lymphoid leukosis, Marek’s disease, mycoplasma air sacculitis, mycoplasmosis, ornithobacteriosis, feather disease, psittacosi, pullorum, reticuloendotheliosis, salmonellosis, turkey hepatitis, swollen head syndrome, white chick syndrome, young pigeon disease syndrome and so on. Detecting the quantity of marker proteins from different samples may benefit from ELISA, Lateral flow immunoassay (LFIA), colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA), turbidimetric inhibition immuno assay (TINIA), immunonephelometry and POCT.