AAV protocol Download Adeno Associated Virus (AAV) Adeno-associated virus (AAV) is a small-single strand DNA virus, member of human parvovirus, originally described in the 1960s by Bob Atchison at Pittsburgh and Wallace Rowe at NIH as a contaminant in preparation of adenovirus [1,2]. Shortly after its discovery, AAV was then isolated from humans, but serological studies […]
Category Archives: gene therapy
AAV-LC3 User Manual Adenovirus-LC3 User Manual Lentivirus-LC3 User Manual
Lentivirus User Manual Lentivirus-LC3 User Manual Lentivirus-crispr User Manual
Adenovirus User Manual Adenovirus-LC3 User Manual CRISPR/Cas9 Knockout Adenovirus User Manual
AAV(adeno associated virus) User Manual AAV-LC3 User Manual AAV-SaCas9 User Manual
Lentivirus Protocol Download a) Introduction of recombinant lentivirus vector system Since wild-type HIV-1 based lentivirus is associated with destruction of host immune system, especially CD4+ helper T lymphocytes, multiple generations of lentivirus vectors have been designed with enhanced safety features and as attractive vectors for gene therapy. To date, there have been three generations of […]
AAV protocol Download AAV capsids evolution and tissue-specific AAV discovery & development service The Genemedi help scientists and researchers in gene therapy vector development. Besides different scales of AAV production, we can also offer novel AAV discovery services with AAV re-engineering capsids library and sub-library generation based on different strategies, customized AAV capsids screening and […]
Lentivirus Protocol Download The life cycle of HIV-1 starts with viral entry, in which process the virus binds to the CD4 receptor or a coreceptor (CCR5 or CXCR4) with gp120 protein, thereby anchoring itself onto host cell surface, allowing fusion between the cellular and viral membranes. After entry into the cell, the viral nucleoprotein together […]
Lentivirus Protocol Download Lentivirus, as represented by HIV-1, is a medium-sized (80-100nm) and enveloped, slightly pleomorphic, spherical virus with an isometric nucleocapsid (Figure1A). Unlike other retroviruses, HIV-1 is featured by a set of additional regulatory and accessory genes [11,12]. Its DNA genome, transcribed from HIV-1 ssRNA, is approximately 9.7 kb and contains 9 ORFs. Besides […]
Lentivirus Protocol Download a) Advantages of lentivirus -mediated gene delivery Lentivirus has been developed as an attractive candidate for creating viral vectors for gene therapy due to various advantages.1) Customized cloning for any other gene ORF expression, shRNA/miRNA and CRISPR/Cas9.2) No known immunogenic proteins generated.3) High titer. 108TU/ml or 109TU/ml lentiviral titer for cell line […]
