diagnostic-Antibiotics

Diagnostic antibodies and antigens for the detection of maximum residue limit(MRL) of antibiotics

Antibiotics are used for veterinary purposes almost as soon as they had been developed for human medicine. They are supplemented in the animal feed for growth promotion and treatment of infections, which are important during the development of intensive methods of animal husbandry, as a result there are major hazards that could be posed by antibiotic residues in foods like raw milk, poultry, cattle, p0rcine, fish and so on. The possible presence of antimicrobial residues (AR) in farm foods poses a risk for consumers such as allergic reactions, toxicity, carcinogenic effects, selection of resistant bacteria, disruption of human normal flora, provoke immunological response and inhibition of the starter culture.

The risk of residue from these foods are higher in developing countries compared to developed one. This might be related with lack of facilities for detection and regulatory bodies that control the drug residues level in foods in the form of maximum residue limits (MRLs). Therefore, to prevent/minimize the risk of antimicrobial residue in milk, different methods of detection of residue to the standard limits level in all food items is possible by chemical methods, microbiological methods and immunological assays. However, Genemedi developed the antigen and antibody to detect the MRL of various antibiotics in the food such as raw milk, poultry, cattle, p0rcine, fish and so on. Detecting the MRL of different antibiotics from different samples may benefit from ELISA, Lateral flow immunoassay (LFIA), colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA), turbidimetric inhibition immuno assay (TINIA), immunonephelometry and POCT.

Maximum Residue Limits (MRLs) for Veternary Drugs in Foods.

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Cat No.
Small molecules (Chemicals, Antibiotics, Mycotoxins, Hormones, Drugs of Abuse, etc.)
Carrier-coupled antigen/immunogen (hapten-carrier conjugates)
Anti-Hapten Antibody
Bioactivity validation
ELISA IC50(ppb)
Order
Sulfonamides Residue (SAs) (sulfamethoxazole (SMX), 5-atom-rings)
BSA-Sulfonamides Residue (SAs) (sulfamethoxazole (SMX), 5-atom-rings); OVA-Sulfonamides Residue (SAs) (sulfamethoxazole (SMX), 5-atom-rings)
Anti-Sulfonamides Residue (SAs) (sulfamethoxazole (SMX), 5-atom-rings) mouse monoclonal antibody
Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
2-5
Chloramphenicol (TAP )
BSA-Chloramphenicol (TAP ); OVA-Chloramphenicol (TAP )
Anti-Chloramphenicol (TAP ) mouse monoclonal antibody
Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
0.2-0.3
Chloramphenicols
BSA-Chloramphenicols; OVA-Chloramphenicols
Anti-Chloramphenicols mouse monoclonal antibody
Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
0.2-0.4
Kanamycin(KAN)
BSA-Kanamycin(KAN); OVA-Kanamycin(KAN)
Anti-Kanamycin(KAN) mouse monoclonal antibody
Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
1.00
Gentamicin(GM)
BSA-Gentamicin(GM); OVA-Gentamicin(GM)
Anti-Gentamicin(GM) mouse monoclonal antibody
Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
0.4-0.5
Amikacin(AMK)
BSA-Amikacin(AMK); OVA-Amikacin(AMK)
Anti-Amikacin(AMK) mouse monoclonal antibody
Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
0.1-0.2
Streptomycin(STR)
BSA-Streptomycin(STR); OVA-Streptomycin(STR)
Anti-Streptomycin(STR) mouse monoclonal antibody
Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
0.4-0.5
Apramycin(APM)
BSA-Apramycin(APM); OVA-Apramycin(APM)
Anti-Apramycin(APM) mouse monoclonal antibody
Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
0.00
Spectinomycin(SPM)
BSA-Spectinomycin(SPM); OVA-Spectinomycin(SPM)
Anti-Spectinomycin(SPM) mouse monoclonal antibody
Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
1.50
Tobramycin
BSA-Tobramycin; OVA-Tobramycin
Anti-Tobramycin mouse monoclonal antibody
Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
5.00
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