Anti-small molecules (Chemicals, Antibiotics£¬Mycotoxins,etc.) antibodies and small molecules competitive antigens (Carrier-coupled antigen, immunogen, hapten-carrier conjugates, BSA-conjugated, OVA-conjugated), competitive ELISA validated

Competitive immunoassay-validated anti-Hapten (small molecules, chemicals) antibody against hapten (small molecules, chemicals) and hapten-carrier conjugated competitive antigens(Carrier-coupled antigen,immunogen,hapten-carrier conjugates, BSA-conjugated, OVA-conjugated).

The anti-Hapten antibodies against haptens had been validated with our hapten-carrier conjugates via competitive ELISA test.

GeneMedi offers paired anti-small molecules (Chemicals, Antibiotics,Mycotoxins,Hormones,Drugs of Abuse, etc.) antibodies (monoclonal antibody, mab) and small molecules competitive antigens (Carrier-coupled antigen, immunogen, hapten-carrier conjugates, BSA-conjugated, OVA-conjugated) for the rapid test and diagnostics kit of Growth Promoters, Toxic Heavy Metal, Myotoxins,Nutritions, Food Safety, Pesticides, Drugs of Abuse, Allergen, Antibiotics, Agricultural, Water Contamination and so on.

All our anti-chemicals antibodies and small molecules hapten-carrier conjugates antigens (BSA-conjugated, OVA-conjugated)are suitable for in competitive ELISA, Lateral flow immunoassay (LFIA) and other immunoassays in diagnostics and rapid test kit. The carrier-coupled antigens of small molecules can act as immunogens.

Trimethoprim is a synthetic derivative of trimethoxybenzyl-pyrimidine with antibacterial and antiprotozoal properties. Sulfamethoxazole with trimethoprim is a fixed antibiotic combination that is widely used for mild-to-moderate bacterial infections and as prophylaxis against opportunistic infections. Recently, our R&D department demonstrated that our GMP-SMT-73-Ab-1 (Anti-Trimethoprim(TMP) mouse monoclonal antibody) has a large linear range and good sensitivity against the GMP-SMT-73-Ag-1 (BSA-Trimethoprim(TMP)). Below is the result of GeneMedi’s Anti-Trimethoprim(TMP) mouse monoclonal antibody validation with BSA-Trimethoprim(TMP) in ELISA. We highly recommend the Ab&Ag to you.

Figure 1. GeneMedi's GMP-SMT-73-Ab-1 (Anti-Trimethoprim(TMP) mouse monoclonal antibody) is validated to detect the GMP-SMT-73-Ag-1 (BSA-Trimethoprim(TMP)) in ELISA. EC50 = 234.5 ng/ml.

Genemedi supplies Small Molecule Diagnostic for a wide selection of choices (please see below).

classification
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Cat No.
Small molecules (Chemicals, Antibiotics, Mycotoxins, Hormones, Drugs of Abuse, etc.)
Carrier-coupled antigen/immunogen (hapten-carrier conjugates)
Anti-Hapten Antibody
Bioactivity validation
ELISA IC50(ppb)
Order
Sulfonamides Residue (SAs) (sulfamethoxazole (SMX), 5-atom-rings)
BSA-Sulfonamides Residue (SAs) (sulfamethoxazole (SMX), 5-atom-rings); OVA-Sulfonamides Residue (SAs) (sulfamethoxazole (SMX), 5-atom-rings)
Anti-Sulfonamides Residue (SAs) (sulfamethoxazole (SMX), 5-atom-rings) mouse monoclonal antibody
Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
2-5
Chloramphenicol (TAP )
BSA-Chloramphenicol (TAP ); OVA-Chloramphenicol (TAP )
Anti-Chloramphenicol (TAP ) mouse monoclonal antibody
Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
0.2-0.3
Acetamiprid(AAP)
BSA-Acetamiprid(AAP); OVA-Acetamiprid(AAP)
Anti-Acetamiprid(AAP) mouse monoclonal antibody
Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
2-3
Nitenpyram(NIT)
BSA-Nitenpyram(NIT); OVA-Nitenpyram(NIT)
Anti-Nitenpyram(NIT) mouse monoclonal antibody
Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
2-3
Carbofuran(CAR)
BSA-Carbofuran(CAR); OVA-Carbofuran(CAR)
Anti-Carbofuran(CAR) mouse monoclonal antibody
Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
0.1-0.3
Aldicarb(ADC)
BSA-Aldicarb(ADC); OVA-Aldicarb(ADC)
Anti-Aldicarb(ADC) mouse monoclonal antibody
Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
0.1-0.3
Fenpropathrin(FPP)
BSA-Fenpropathrin(FPP); OVA-Fenpropathrin(FPP)
Anti-Fenpropathrin(FPP) mouse monoclonal antibody
Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
3-5
Isoprocarb
BSA-Isoprocarb; OVA-Isoprocarb
Anti-Isoprocarb mouse monoclonal antibody
Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
1-2
Fipronil 
BSA-Fipronil ; OVA-Fipronil 
Anti-Fipronil  mouse monoclonal antibody
Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
0.60
2,4,5-Trichlorophenoxyacetic acid(2,4,5-T)
BSA-2,4,5-Trichlorophenoxyacetic acid(2,4,5-T); OVA-2,4,5-Trichlorophenoxyacetic acid(2,4,5-T)
Anti-2,4,5-Trichlorophenoxyacetic acid(2,4,5-T) mouse monoclonal antibody
Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
3-5
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Disease classification
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Cat No.
Species
Biomarker
Cat No.of Antigen
Bioactivity validation of Antigen
Cat No.of Antibodies
Bioactivity validation of Antibodies
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Human
25 hydroxyvitamin D (25-OH-(VD3+VD2))
GMP-SMT-25-OH-(VD3+VD2)Ag01:BSA-25 hydroxyvitamin D (25-OH-(VD3+VD2)) ;GMP-SMT-25-OH-(VD3+VD2)Ag02: OVA-25 hydroxyvitamin D (25-OH-(VD3+VD2))
Competitive immunoassay-validated hapten-carrier conjugates BSA-& OVA-25 hydroxyvitamin D (25-OH-(VD3+VD2)), 25 hydroxyvitamin D (25-OH-(VD3+VD2)) antibodies binding, Immunogen in Competitive immunoassay validation, Elisa, lateral-flow tests, and other immunoassays as control material in 25-OH-(VD3+VD2) level test of Metabolic diseases (bone fracture, coronary heart disease, cancer, and all-cause mortality) and related syndrome evaluation.
GMP-SMT-25-OH-(VD3+VD2)-Ab01
Anti-25 hydroxyvitamin D (25-OH-(VD3+VD2)) mouse monoclonal antibody (mAb)
Human 25 hydroxyvitamin D (25-OH-(VD3+VD2)) binding, in Competitive immunoassay validation, Elisa, lateral-flow tests, and other immunoassays in 25-OH-(VD3+VD2) level test of Metabolic diseases (bone fracture, coronary heart disease, cancer, and all-cause mortality) and related syndrome evaluation.
Human
25-hydroxy (OH) Vitamin (25-OH-VD)
GMP-SMT-25-OH-VDAg01:BSA-25-hydroxy (OH) Vitamin (25-OH-VD) ;GMP-SMT-25-OH-VDAg02: OVA-25-hydroxy (OH) Vitamin (25-OH-VD)
Competitive immunoassay-validated hapten-carrier conjugates BSA-& OVA-25-hydroxy (OH) Vitamin (25-OH-VD), 25-hydroxy (OH) Vitamin (25-OH-VD) antibodies binding, Immunogen in Competitive immunoassay validation, Elisa, lateral-flow tests, and other immunoassays as control material in 25-OH-VD level test of Metabolic diseases (osteoporosis?and?rickets) and related syndrome evaluation.
GMP-SMT-25-OH-VD-Ab01
Anti-25-hydroxy (OH) Vitamin (25-OH-VD) mouse monoclonal antibody (mAb)
Human 25-hydroxy (OH) Vitamin (25-OH-VD) binding, in Competitive immunoassay validation, Elisa, lateral-flow tests, and other immunoassays in 25-OH-VD level test of Metabolic diseases (osteoporosis and rickets) and related syndrome evaluation.
Human
GMP-SMT-Bisphenol-AAg01:BSA-Bisphenol A ;GMP-SMT-Bisphenol-AAg02: OVA-Bisphenol A
Competitive immunoassay-validated hapten-carrier conjugates BSA-& OVA-Bisphenol A, Bisphenol A antibodies binding, Immunogen in Competitive immunoassay validation, Elisa, lateral-flow tests, and other immunoassays as control material in Bisphenol A level test of Hormone disorders (Toxicity, mutagen and carcinogen) and related syndrome evaluation.
GMP-SMT-Bisphenol-A-Ab01
Anti-Bisphenol A mouse monoclonal antibody (mAb)
Human Bisphenol A binding, in Competitive immunoassay validation, Elisa, lateral-flow tests, and other immunoassays in Bisphenol A level test of Hormone disorders (Toxicity, mutagen and carcinogen) and related syndrome evaluation.
Human
GMP-SMT-CalcitoninAg01:BSA-Calcitonin ;GMP-SMT-CalcitoninAg02: OVA-Calcitonin
Competitive immunoassay-validated hapten-carrier conjugates BSA-& OVA-Calcitonin, Calcitonin antibodies binding, Immunogen in Competitive immunoassay validation, Elisa, lateral-flow tests, and other immunoassays as control material in Calcitonin level test of Hormone disorders (thyroid cancer) and related syndrome evaluation.
GMP-SMT-Calcitonin-Ab01
Anti-Calcitonin mouse monoclonal antibody (mAb)
Human Calcitonin binding, in Competitive immunoassay validation, Elisa, lateral-flow tests, and other immunoassays in Calcitonin level test of Hormone disorders (thyroid cancer) and related syndrome evaluation.
Human
cyclic citrullinated peptide (CCP)
GMP-SMT-CCPAg01:BSA-cyclic citrullinated peptide (CCP) ;GMP-SMT-CCPAg02: OVA-cyclic citrullinated peptide (CCP)
Competitive immunoassay-validated hapten-carrier conjugates BSA-& OVA-cyclic citrullinated peptide (CCP), cyclic citrullinated peptide (CCP) antibodies binding, Immunogen in Competitive immunoassay validation, Elisa, lateral-flow tests, and other immunoassays as control material in CCP level test of Inflammation/autoimmune/inflammatory disease (rheumatoid arthritis) and related syndrome evaluation.
GMP-SMT-CCP-Ab01
Anti-cyclic citrullinated peptide (CCP) mouse monoclonal antibody (mAb)
Human cyclic citrullinated peptide (CCP) binding, in Competitive immunoassay validation, Elisa, lateral-flow tests, and other immunoassays in CCP level test of Inflammation/autoimmune/inflammatory disease (rheumatoid arthritis) and related syndrome evaluation.
Human
Cholyglycine (CG)
GMP-SMT-CGAg01:BSA-Cholyglycine (CG) ;GMP-SMT-CGAg02: OVA-Cholyglycine (CG)
Competitive immunoassay-validated hapten-carrier conjugates BSA-& OVA-Cholyglycine (CG), Cholyglycine (CG) antibodies binding, Immunogen in Competitive immunoassay validation, Elisa, lateral-flow tests, and other immunoassays as control material in CG level test of Liver diseases (liver cirrhosis or obstructive liver disease) and related syndrome evaluation.
GMP-SMT-CG-Ab01
Anti-Cholyglycine (CG) mouse monoclonal antibody (mAb)
Human Cholyglycine (CG) binding, in Competitive immunoassay validation, Elisa, lateral-flow tests, and other immunoassays in CG level test of Liver diseases (liver cirrhosis or obstructive liver disease) and related syndrome evaluation.
Human
deoxycholic acid (cholanoic acid)
GMP-SMT-cholanoic acidAg01:BSA-deoxycholic acid (cholanoic acid) ;GMP-SMT-cholanoic acidAg02: OVA-deoxycholic acid (cholanoic acid)
Competitive immunoassay-validated hapten-carrier conjugates BSA-& OVA-deoxycholic acid (cholanoic acid), deoxycholic acid (cholanoic acid) antibodies binding, Immunogen in Competitive immunoassay validation, Elisa, lateral-flow tests, and other immunoassays as control material in cholanoic acid level test of Liver diseases (liver cirrhosis, chronic kidney disease, vascular calcification, vasculature toxicity) and related syndrome evaluation.
GMP-SMT-cholanoic acid-Ab01
Anti-deoxycholic acid (cholanoic acid) mouse monoclonal antibody (mAb)
Human deoxycholic acid (cholanoic acid) binding, in Competitive immunoassay validation, Elisa, lateral-flow tests, and other immunoassays in cholanoic acid level test of Liver diseases (liver cirrhosis, chronic kidney disease, vascular calcification, vasculature toxicity) and related syndrome evaluation.
Human
GMP-SMT-CortisolAg01:BSA-Cortisol ;GMP-SMT-CortisolAg02: OVA-Cortisol
Competitive immunoassay-validated hapten-carrier conjugates BSA-& OVA-Cortisol, Cortisol antibodies binding, Immunogen in Competitive immunoassay validation, Elisa, lateral-flow tests, and other immunoassays as control material in Cortisol level test of Hormone disorders (adrenal gland disorder) and related syndrome evaluation.
GMP-SMT-Cortisol-Ab01
Anti-Cortisol mouse monoclonal antibody (mAb)
Human Cortisol binding, in Competitive immunoassay validation, Elisa, lateral-flow tests, and other immunoassays in Cortisol level test of Hormone disorders (adrenal gland disorder) and related syndrome evaluation.
Human
GMP-SMT-DES-HESAg01:BSA-Diethylstilbestrol/Hexestrol (DES/HES) ;GMP-SMT-DES-HESAg02: OVA-Diethylstilbestrol/Hexestrol (DES/HES)
Competitive immunoassay-validated hapten-carrier conjugates BSA-& OVA-Diethylstilbestrol/Hexestrol (DES/HES), Diethylstilbestrol/Hexestrol (DES/HES) antibodies binding, Immunogen in Competitive immunoassay validation, Elisa, lateral-flow tests, and other immunoassays as control material in DES/HES level test of Hormone disorders (Food safety) and related syndrome evaluation.
GMP-SMT-DES-HES-Ab01
Anti-Diethylstilbestrol/Hexestrol (DES/HES) mouse monoclonal antibody (mAb)
Human Diethylstilbestrol/Hexestrol (DES/HES) binding, in Competitive immunoassay validation, Elisa, lateral-flow tests, and other immunoassays in DES/HES level test of Hormone disorders (Food safety) and related syndrome evaluation.
Human
GMP-SMT-DEXAg01:BSA-Dexamethasone (DEX) ;GMP-SMT-DEXAg02: OVA-Dexamethasone (DEX)
Competitive immunoassay-validated hapten-carrier conjugates BSA-& OVA-Dexamethasone (DEX), Dexamethasone (DEX) antibodies binding, Immunogen in Competitive immunoassay validation, Elisa, lateral-flow tests, and other immunoassays as control material in DEX level test of Hormone disorders (Cushing's syndrome) and related syndrome evaluation.
GMP-SMT-DEX-Ab01
Anti-Dexamethasone (DEX) mouse monoclonal antibody (mAb)
Human Dexamethasone (DEX) binding, in Competitive immunoassay validation, Elisa, lateral-flow tests, and other immunoassays in DEX level test of Hormone disorders (Cushing's syndrome) and related syndrome evaluation.
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Antibiotics

Diagnostic antibodies and antigens for the detection of maximum residue limit(MRL) of antibiotics

Antibiotics are used for veterinary purposes almost as soon as they had been developed for human medicine. They are supplemented in the animal feed for growth promotion and treatment of infections, which are important during the development of intensive methods of animal husbandry, as a result there are major hazards that could be posed by antibiotic residues in foods like raw milk, poultry, cattle, p0rcine, fish and so on. The possible presence of antimicrobial residues (AR) in farm foods poses a risk for consumers such as allergic reactions, toxicity, carcinogenic effects, selection of resistant bacteria, disruption of human normal flora, provoke immunological response and inhibition of the starter culture.

The risk of residue from these foods are higher in developing countries compared to developed one. This might be related with lack of facilities for detection and regulatory bodies that control the drug residues level in foods in the form of maximum residue limits (MRLs). Therefore, to prevent/minimize the risk of antimicrobial residue in milk, different methods of detection of residue to the standard limits level in all food items is possible by chemical methods, microbiological methods and immunological assays. However, Genemedi developed the antigen and antibody to detect the MRL of various antibiotics in the food such as raw milk, poultry, cattle, p0rcine, fish and so on. Detecting the MRL of different antibiotics from different samples may benefit from ELISA, Lateral flow immunoassay (LFIA), colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA), turbidimetric inhibition immuno assay (TINIA), immunonephelometry and POCT.

Food Safety

Agricultural

Allergen

Mycotoxins

Drugs of Abuse

Toxic Heavy Metal

Hormones

Chemicals

Vitamins

Amino Acids

IVD

Introdution-Food safety and small molecule detection

Food can transmit disease from person to person by serving as a growth medium for food contaminations that can cause food poisoning. Food safety refers to non-toxic, harmless of food, in line with the nutritional requirements, and does not cause any acute, subacute or chronic hazards on human health. Currently, food safety is a growing concern all over the world especially in developing areas. Ensuring the safety of food has become a significant challenge due to globalization of the food supply and the demand for minimally processed food products. Some potential sources for chemical and biological contamination of foods include: the use of pesticides, fertilizers, and antibiotics around agricultural food products; accidental introduction of additives to industrially processed foods at unsafe levels, or intentional adulteration with low quality or unsafe ingredients for economic purposes; cross-contamination with allergens or other substances that can be dangerous to sensitive individuals (e.g., wheat gluten); microbial growth deriving from unsanitary agricultural or processing conditions; and spoilage during transport and storage of foods due to packaging defects or incorrect handling by consumers. To manage food safety risks, there is a continued need for rapid, sensitive, inexpensive, and reliable techniques to detect the presence of chemical contaminants and microbes in complex media.

Food Safety Solutions

Food testing and analyzing plays an important role in quality control of food production.

1. Culture-based traditional techniques

Traditional culture methods use selective liquid or solid culture media to grow, isolate, and enumerate target microorganism and simultaneously prevent the growth of other microorganisms present in the food (Jasson et al., 2010). These methods for the identification of foodborne pathogens involve pre-enrichment growth, selective enrichment culture, and selective plating followed by biochemical identification and serological confirmation of results. These methods are relatively inexpensive, sensitive, and still regarded as gold standards, the main drawback of these methods is their long analysis time and labor intensiveness. The whole procedure takes typically between 7 and 10 days (Vunrcrzant and Pllustoesser, 1987; Biswas, 2005).

2. Mircoscopic and optical characteristics based methods

Various methods based on microscopic and optical characteristics of the appropriately stained microbial cells have been developed for assuring microbial safety of foods and food products. These methods include Direct Epifluorescent Filter Technique (DEFT), Flow Cytometry, and Solid Phase Cytometry. DEFT is a rapid method for enumerating microbial foodborne pathogens and is used widely in the dairy industry for raw foods (Hermida et al., 2000), milk and milk products, beverages, foods, etc. Flow cytometry is applied for the enumeration of viable bacteria in a sample, and uses flourescent dyes for the analysis of viability, metabolic state, and antigenic markers of bacteria. Solid-phase cytometry (SPC) combines the principles of epifluorescence microscopy and flow cytometry.

3. Immunological methods

All immunological methods for the detection of foodborne pathogens are based on antigen–antibody reactions. The body produces specific antibodies in response to invading pathogen. These reactions are versatile and specific but the success of an immunoassay depends on the specificity of antibody. Various kits have been developed based on immunological methods for the detection of foodborne pathogens, and are available commercially and have been responsible for revolutionizing the field of food testing (Table 1).

4. Nucleic acid-based methods

Spurred by technological developments and commercial profit motives, nucleic acid-based assays have become widely available as powerful tools to assist in the diagnosis and monitoring of foodborne pathogens. These methods are based on the detection of specific gene sequences (signature sequences) in the genotype of target organism. The sequences may be selected in such a way that they can detect a particular group, genus, species, or even the strain of microorganism. There are many DNA-based assay formats, but probes and nucleic acid amplification techniques are the most popular ones and have been developed commercially for detecting foodborne pathogens.

5. Mass spectrometry

For years, mass spectrometry has been considered the most suitable analytical technique for the detection of multiple compounds in food, feed and water. Coupled to liquid chromatography (LC), high-performance LC and ultra-high performance LC (HPLC, UHPLC) or gas chromatographic (GC) separation with an ionization source such as electrospray (ESI), a large number of mass spectrometry-based methods were developed to comply with updated regulations. To effectively apply this approach, the structure of the compound must be characterized before its detection. Methods development can be time-consuming, and standards must be acquired to optimize compound-specific instrumental conditions, including transition selections, ion-source voltages, and collision energies. MRM methods are, therefore, unable to screen for unknown compounds

Small molecule detection-Hapten based competitive immunoassay

Small molecules such as pesticides, drugs, etc. are usually nonimmunogenic and hence do not elicit an immune response unless coupled with some macromolecules such as proteins. It is, therefore, required to modify these small substances (hapten) for coupling with macromolecules (carrier) so as to make a stable carrierhapten complex. Synthesis of hapten for linking with carrier proteins is the most important aspect of specific antibody generation against small molecules for immunoassay applications.

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