Lentivirus FAQs 1. What is a Lentivirus? Lentivirus is a subfamily of the retrovirus family. Lentiviruses can deliver significant amounts of genetic information into host cells and integrate it into the cellular genome. Genetically-engineered lentiviruses are therefore used as one of the most efficient tools of gene delivery.These lentiviruses contain a viral promoter which is […]
1. How many cells should there be in a well? For adhesive cells, at least 1000 cells are required per well (100 μl medium) when using the kit’s standard 96-well plate. For leukocytes, at least 2500 cells are necessary per well (100 μl medium) because of low sensitivity. The recommended maximum number of cells per […]
1. What are Mycoplasmas? Mycoplasmas are very small bacteria lacking cell walls that belong to various genera within the class Mollicutes. They are flexible, pleomorphic organisms that can be as small as 0.2 to 0.3. 2. What are the consequences of mycoplasma contamination? Because of their typically high concentrations in cell cultures, mycoplasmas can often […]
Q1. Are cell density (% confluence) and passage number important considerations for transfection? A: Yes, cell density is an important parameter in influencing transfection efficiency. If the seeding density is too low, some cytotoxicity may be observed. If the cell density is high, lower than expected transfection efficiency may be observed. Both issues may be […]
1. Why does adenovirus have a relatively higher immunogenecity compared with rAAV? The adenovirus without E1/E3 can express all of the other genes in the viral backbone and hence induces immunogenic responses, while rAAV does not have any of the AAV genes, thus no immunogenicity from viral protein. 2. What is the role of the […]
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Q1. How to calculate the Multiplicity of infection (MOI)? I have to do transduction using lentivirus and I have a doubt how to calculate the MOI. I have 5×10 3 ( 5000 viral particles/ ul). How much do I have to take to get MOI of 1 if I plate 10000 cells in a 12 […]
1. Why do you offer GeneGelRed in DMSO or water? The water formulation is a newer and improved product compared to the stock in DMSO. We recommend using dyes in water to avoid the potential hazards of handling DMSO, which can be absorbed through the skin. We continue to offer dyes in DMSO because some […]
1. What is the maximum length of the DNA fragment assembled? Gibson Assembly Cloning Kit has been used to clone a 15 kb DNA fragment into a 5 4 kb plasmid in E. coli, totaling up to 20 4 kb in length 2. How many fragments of DNA can I assemble in one reaction? The […]
1. What is the recommended dilution range for Femto Signal Western ECL Substrate? Primary antibody recommended dilution range is 1 to 0.2 µg/mL primary antibody (1:5,000 to 1:100,000 dilution from 1µg/mL stock). Secondary antibody recommended dilution range is 2 to 10 ng/mL secondary antibody (1:100,000 to 1:500,000 dilution from µg/mL stock). 2. What is the […]
