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Adenovirus Specific for Suspension Cells
Introduction to Adenovirus Specific for Suspension Cells
The adenoviral vectors provided by Genemedi are based on human adenovirus type 5 (Ad5), which is replication-incompetent (-E1/-E3) and can’t be integrated into host genome, guaranteeing the security for subsequent operations. Adv packaging by Genemedi shows almost 100% gene delivery in most cell types in the recombinant protein expression system both in vivo and in vitro. Recombinant protein expression of interest is detectable from 24 hours post infection. Furthermore, Genemedi has also developed adenovirus specific for suspension cells, which is modified according to the characteristics of suspension cell membrane. Besides, the virus infection system has also been optimized to obviously increase the infectivity specific for suspension cells. Nevertheless, this virus can also be used to transduce adherent cells, for its infectivity to adherent cells has not changed.
Properties
| Adenovirus Virus | |
|---|---|
| Quantity/Unit | Vials |
| Form | Frozen form |
| Sipping and Storage Guidelines | Shipped by dry ice, stored at -80 ° C, effective for 1 year. Avoid repeatedly freezing and thawing |
| Titer | > 1*10^10 PFU/ml |
Advantages
1. Specific for suspension cells.
2. Extremely high infection efficiency. Adenovirus specific for suspension cells in Genemedi can infect many suspension cells, with almost 100% infection efficiency for Jurat and K562. As for primary cells derived from blood, such as CD4+ T cells, the infection efficiency will be more than 60%.
3. Great packaging capacity, up to 8kb.
4. Without integration. No alteration to host genome.
5. High titer. Up to 1011 pfu/ml.
Applications and Figures
Quality control description
Our optimized custom adenoviral vector production and strict quality control systems provide customers a high titer of functional recombinant adenoviral vectors. Viral titers are determined by TCID50 method, which is the most accurate way to measure the titer of adenovirus.
Technical Documents
1. For detailed protocol about how to package and purify Adv, please see the 
Adenovirus User Manual on the Genemedi website.
Adenovirus User Manual on the Genemedi website. Reference
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