Category Archives: gene therapy

Tissue-specific Cre mouse + AAV-DIO-GOI for tissue-specific gene overexpression |-GeneMedi

View Knowledge Base – Cre-loxp system and Viral vector (AAV and adenovirus)-based Cre tools (AAV-Cre and Ad-Cre)>> Product list: Cre/loxP tools in AAV vector(AAV-Cre), adenoviral vector(Ad-Cre) and lentiviral vector(Lv-Cre) Abstract Cre-loxP system is widely used in the field of biosciences, especially in the generation of genetically engineered mice (knockout or overexpression), enabling researchers to study […]

 Inducible-tissue specific Cre-loxP system for in vivo and in vitro study

View Knowledge Base – Cre-loxp system and Viral vector (AAV and adenovirus)-based Cre tools (AAV-Cre and Ad-Cre)>> Product list: Cre/loxP tools in AAV vector(AAV-Cre), adenoviral vector(Ad-Cre) and lentiviral vector(Lv-Cre) Abstract Cre-loxP system is widely used in the field of biosciences, especially in the generation of genetically engineered mice (knockout or overexpression), enabling researchers to study […]

Landscape of in vitro cell transfection and in vivo transfection (siRNA transfection,DNA/plasmid transfection, nanoparticle transfecton, electroporation, transfection protocol and so on)

Transfection protocol Download Introduction of transfection In general, transfection is the process of delivering nucleic acids, such as DNA and RNA into eukaryotic cells, resulting in the expression or production of proteins or down-regulation of the targeted protein. Although not very common, protein transfection is also used to promote the rapid expression of target protein […]

Lentivirus FAQs

Lentivirus FAQs 1. What is a Lentivirus? Lentivirus is a subfamily of the retrovirus family. Lentiviruses can deliver significant amounts of genetic information into host cells and integrate it into the cellular genome. Genetically-engineered lentiviruses are therefore used as one of the most efficient tools of gene delivery.These lentiviruses contain a viral promoter which is […]

AAV Vector System (AAV packaging and expression system)

AAV protocol Download AAV User Manual Download AAV vector system (AAV expression system, AAV packaging plasmid system)-Introduction GeneMedi’s AAV Vector System, also named AAV expression system or AAV packaging plasmid system, is powerful tool for in-vivo gene delivery, gene editing and gene therapy. You can easily produce recombinant AAV (rAAV) paticle in 293T cell line […]

CRISPR/Cas9 Knockout Lentivirus Production Service

Introduction to CRISPR/Cas9 Knockout Lentivirus Production Service CRISPR/Cas9 premade products Custom made CRISPR/Cas9 service Custom-made CRISPR/Cas9-gRNA lentivirus for knockout cell line development Custom-made CRISPR/Cas9-gRNA adenovirusfor knockout cell line development Custom-made CRISPR/Cas9-gRNA AAVfor tissue-specific knockout in vivo CRISPR/Cas9 User Manual

Lentivirus-LC3 production service for Autophagy Flux Detection

Customized Lentivirus production Introduction to AAV-LC3 production service for Autophagy Flux Detection Premade LC3 Autophagy Biosensors Products and user manual Adeno associated virus(AAV) AAV-GFP-LC3 Autophagy Biosensor AAV-mRFP-GFP-LC3 Autophagy Biosensor Adenovirus Adv-GFP-LC3 Autophagy Biosensor Adv-mRFP-GFP-LC3 Autophagy Biosensor Lentivirus Lv-GFP-LC3 Autophagy Biosensor Lv-mRFP-GFP-LC3 Autophagy Biosensor LC3 Autophagy Biosensors User Manual AAV-LC3 User Manual    Adenovirus-LC3 User Manual  […]

Custom Lentivirus Production Service

Begin your customized Lentivirus production process Introduction to Pre-made Lentivirus Production Service Lentivirus is a powerful tool for delivering target genes into almost all types of mammalian cells in vitro and in vivo. In contrast to retroviruses, lentiviruses are imported much more actively into the nuclei of non-dividing cells and are stably integrated into the […]

AAV vector system, AAV production, transduction and AAV gene therapy

Adeno-associated virus (AAV vector) -Introduction Landscape, protocol and guidelines of Adeno-associated virus (AAV) vector system, AAV production,AAV transduction and AAV gene therapy are described below. The detail information is mentioned about AAV vector plasmids cloning, AAV packaging, purification, AAV capsid evolution and insight of AAV gene therapy. Table of Contents Adeno-associated virus (AAV vector) and […]