Adenovirus vector system (adenovirus expression system, adenovirus packaging plasmid system)

Introduction to Adenovirus vector system

GeneMedi’s adenovirus Vector System, also named the adenovirus expression system or adenovirus packaging plasmid system, is a powerful tool for in-vitro & in-vivo gene delivery, shRNA mediated RNA interference (RNAi) and gene editing. You can easily recombine produce a recombinant adenovirus (r-AdV) particle in HEK293 or HEK293A cell line in high titer using GeneMedi’s adenovirus Vector System.

The Genemedi adenovirus vector system including 2 types of adenovirus packaging plasmids: the adenovirus expression plasmids (adenovirus shuttle vectors) with different promoters and tags, and an adenovirus genome backbone plasmid with E1 region deletion.

Figure 1: Workflow of adenovirus packaging


Shuttle Vector Construction and Preparation       Backbone Vector Preparation

Transfection in 293A Cells

Individual Plaques Picking and Infection of 293A Cells

Freeze-Thaw Cycles on Infected Cells and Supernatent

Re-infection of 293A Cells with rAdV in Collected in Freeze-Thaw Cycles

Isolation, Purification and Titration of rAdV

Transduction of Target Cells

GeneMedi has developed a variety of adenovirus expression vectors with different expression cassettes, containing kinds of verified promoters and reporters including GFP, zsgreen, RFP, mcherry and luciferase. The GeneMedi’s adenovirus expression vectors have been proved very suitalble for unique gene overexpression or shRNA-mediated knock-down (also called RNAi (RNA interference ). You can also achieve gene knock-out(KO) or gene editing using our Crispr-cas9-gRNA adenovirus expression vector.

Introduction to Adenovirus Vector System (Adenovirus packaging and expression system)

Finish adenovirus packaging in 2 weeks with Genemedi’s FasterAd Adenovirus vector system (adenovirus packaging system). The Genemedi’s FasterAd Adenovirus packaging system using a HEK293 recombination system including the adenovirus expression vector (adenovirus overexpression or shRNA vector) and the adenovirus packaging plasmid (adenovirus backbone).

Recombinant adenovirus (Adenovirus), a replication-defective adenoviral vector system, is widely used for gene delivery in most cell types. The adenoviral vectors provided by Genemedi are based on human adenovirus type 5 (Ad5), which is replication-incompetent (-E1/-E3) and can’t be integrated into host genome, guaranteeing the security for subsequent operations. Adenovirus packaging by Genemedi shows almost 100% gene delivery in most cell types in the recombinant protein expression system both in vivo and in vitro. Recombinant protein expression of interest is detectable from 24 hours post infection.

An adenovirus packaging system including the adenovirus expression vector and the adenovirus package plasmid. In order to meet different infection needs, Genemedi has launched a variety of adenovirus packaging system plasmids with different fluorescent labels (such as GFP, RFP, Luciferase and so on), and various promoters or protein tags, which may also allow researchers to produce lentiviruses by themselves as they need.

Adenovirus vector system

Adenovirus expression vectors (adenovirus expression plasmids)

Note: Industry R&D excludes CRO&CDMO&CXO; Others includes CRO&CDMO&CXO&CMC&Manufacturing company

Adenovirus genome backbone plasmid


1. Broad range of host. Adenovirus has the ability to infect dividing, quiescent cells, stem cells, and primary cells, allowing genetic materials to be delivered to a highly diverse range of cell types and tissues.

2. High infection efficiency. Almost 100% gene delivery in most cell types, completely surpassing other viral vector tools and liposome transfection.

3. Great packaging capacity, up to 8kb.

4. Without integration. No alteration to host genome.

5. High titer. Up to 1011 PFU/ml.

Applications and Figures

Figure 1 Transduction results of various Adenovirus vectors. Selected results of Adenovirus transduction in the adult rat cardiac cells (A), eyes (B), neuron cells (C).

Technical Documents

1. For detailed protocol about how to package and purify adenovirus, please see the pdf downloadAdenovirus User Manual.


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