No products in the cart.
Pre-made Adenovirus Production And Pre-made Adenovirus particle)
Begin your customized ADV production process
Introduction to Pre-made Adenovirus Production And Pre-made Adenovirus particle)
Besides various adenoviral expression systems used for altering gene expression or monitoring gene expression, more than 10 thousand adenoviral ORF plasmids, which have been confirmed by sequencing, are available for direct Adv packaging.
The adenoviral vectors provided by Genemedi are based on human adenovirus type 5 (Ad5), which is replication-incompetent (-E1/-E3) and can’t be integrated into host genome, guaranteeing the security for subsequent operations. Adv packaging by Genemedi shows almost 100% gene delivery in most cell types in the recombinant protein expression system both in vivo and in vitro.
Genemedi has launched a comprehensive Adv packaging service. Besides various adenoviral expression systems used for altering gene expression or monitoring gene expression, more than 10 thousand transgene plasmids, which have been confirmed by sequencing, are available for direct Adv packaging, which may greatly shorten the supply period of Adv virus, saving more than 3 weeks.
Properties
| Adenovirus Vector | |
|---|---|
| Quantity/Unit | Vials |
| Form | Frozen form |
| Suitable Types of Infection | In vivo infection in animals |
| Sipping and Storage Guidelines | Shipped by dry ice, stored at -80 ℃, effective for 1 year. Avoid repeatedly freezing and thawing. |
| Titer | > 1*10^10 TU/ml. |
Advantages
1. Broad range of host. Adv has the ability to infect dividing, quiescent cells, stem cells, and primary cells, allowing genetic materials to be delivered to a highly diverse range of cell types and tissues.
2. High infection efficiency. As high as 100% gene delivery in most cell types, completely surpassing other viral vector tools and liposome transfection.
3. Great packaging capacity. up to 8kb.
4. Without integration. No alteration to host genome.
5. High titer. Up to 1011 pfu/ml.
6. Short supply period. More than 10 thousand transgene plasmids are available for direct Adv packaging which may save 3 weeks than customized products.
Applications and Figures
Quality control description
Our optimized custom adenoviral vector production and strict quality control systems provide customers a high titer of functional recombinant adenoviral vectors. Viral titers are determined by TCID50 method, which is the most accurate way to measure the titer of adenovirus.
Technical Documents
1. For detailed protocol about how to package and purify Adv, please see the 
Adenovirus User Manual on the Genemedi website.
Adenovirus User Manual on the Genemedi website. Reference
1.Hu J, L Zhang, Y Yang, Y Guo, Y Fan, M Zhang, W Man, E Gao, W Hu, RJ Reiter, H Wang and D Sun. (2017). Melatonin alleviates postinfarction cardiac remodeling and dysfunction by inhibiting Mst1. J Pineal Res 62.
2.Xu C, F Wu, C Mao, X Wang, T Zheng, L Bu, X Mou, Y Zhou, G Yuan, S Wang and Y Xiao. (2016). Excess iodine promotes apoptosis of thyroid follicular epithelial cells by inducing autophagy suppression and is associated with Hashimoto thyroiditis disease. J Autoimmun 75:50-57.
3.Wang Y, X Zhao, X Wu, Y Dai, P Chen and L Xie. (2016). microRNA-182 Mediates Sirt1-Induced Diabetic Corneal Nerve Regeneration. Diabetes 65:2020-31.
4.Luo T, J Fu, A Xu, B Su, Y Ren, N Li, J Zhu, X Zhao, R Dai, J Cao, B Wang, W Qin, J Jiang, J Li, M Wu, G Feng, Y Chen and H Wang. (2016). PSMD10/gankyrin induces autophagy to promote tumor progression through cytoplasmic interaction with ATG7 and nuclear transactivation of ATG7 expression. Autophagy 12:1355-71.
5.Liu TY, XQ Xiong, XS Ren, MX Zhao, CX Shi, JJ Wang, YB Zhou, F Zhang, Y Han, XY Gao, Q Chen, YH Li, YM Kang and GQ Zhu. (2016). FNDC5 Alleviates Hepatosteatosis by Restoring AMPK/mTOR-Mediated Autophagy, Fatty Acid Oxidation, and Lipogenesis in Mice. Diabetes 65:3262-3275.
6.Liu H, S Fang, W Wang, Y Cheng, Y Zhang, H Liao, H Yao and J Chao. (2016). Macrophage-derived MCPIP1 mediates silica-induced pulmonary fibrosis via autophagy. Part Fibre Toxicol 13:55.
7.Yao T, X Ying, Y Zhao, A Yuan, Q He, H Tong, S Ding, J Liu, X Peng, E Gao, J Pu and B He. (2015). Vitamin D receptor activation protects against myocardial reperfusion injury through inhibition of apoptosis and modulation of autophagy. Antioxid Redox Signal 22:633-50.
8.Wang B, A Ma, L Zhang, WL Jin, Y Qian, G Xu, B Qiu, Z Yang, Y Liu, Q Xia and Y Liu. (2015). POH1 deubiquitylates and stabilizes E2F1 to promote tumour formation. Nat Commun 6:8704.
9.Hua F, K Li, JJ Yu, XX Lv, J Yan, XW Zhang, W Sun, H Lin, S Shang, F Wang, B Cui, R Mu, B Huang, JD Jiang and ZW Hu. (2015). TRB3 links insulin/IGF to tumour promotion by interacting with p62 and impeding autophagic/proteasomal degradations. Nat Commun 6:7951.
10.Wang X, J Liu, J Zhen, C Zhang, Q Wan, G Liu, X Wei, Y Zhang, Z Wang, H Han, H Xu, C Bao, Z Song, X Zhang, N Li and F Yi. (2014). Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy. Kidney Int 86:712-25.
