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Pre-made biosensors & Organelle Bioprobe in adenovirus & AAV vector
Pre-made biosensors & Organelle Bioprobe in adenovirus & AAV vector
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Cat No. | Name of Biosensors (BioProbes) | Biosensors/Bioprobes Description
|
|---|---|---|
GM-Probe-Adsensor-01 |
Adv-actin indicator (mTurquoise reporter) |
Actin cytoskeletal localization |
GM-Probe-Adsensor-02 |
Adv-GCaMP6m (medium variant calcium indicator) |
GcaMP6 is a super-sensitive fluorescent protein, which is widely used to reflect the changes of intracellular free Ca2+ concentration. GcaMP6 fluorescent protein can be used as a hypersensitive Ca2+ probe to study the effects of external factors on Ca2+ in living cells under physiological and pathological conditions.GCaMP6m is a genetically encoded fluorescent Ca[2+] indicator that shows high sensitivity and medium decay kinetics. It consists of the calmodulin-binding peptide M13, a circularly permuted green fluorescent protein and calmodulin. |
GM-Probe-Adsensor-03 |
Adv-GCaMP6s (slow variant calcium indicator) |
GcaMP6 is a super-sensitive fluorescent protein, which is widely used to reflect the changes of intracellular free Ca2+ concentration. GcaMP6 fluorescent protein can be used as a hypersensitive Ca2+ probe to study the effects of external factors on Ca2+ in living cells under physiological and pathological conditions.GCaMP6m is a genetically encoded fluorescent Ca[2+] indicator that shows high sensitivity and medium decay kinetics. It consists of the calmodulin-binding peptide M13, a circularly permuted green fluorescent protein and calmodulin. |
GM-Probe-Adsensor-04 |
Adv-GCaMP6f (fast variant calcium indicator) |
GcaMP6 is a super-sensitive fluorescent protein, which is widely used to reflect the changes of intracellular free Ca2+ concentration. GcaMP6 fluorescent protein can be used as a hypersensitive Ca2+ probe to study the effects of external factors on Ca2+ in living cells under physiological and pathological conditions.GCaMP6m is a genetically encoded fluorescent Ca[2+] indicator that shows high sensitivity and medium decay kinetics. It consists of the calmodulin-binding peptide M13, a circularly permuted green fluorescent protein and calmodulin. |
GM-Probe-Adsensor-05 |
Adv-Peroxisome indicator (mTurquoise reporter) |
Peroxisome localization |
GM-Probe-Adsensor-06 |
Adv-lactate biosensor (Laconic fluorescent protein) |
Laconic fluorescent protein can specifically and efficiently reflect the changes of lactate concentration in living cells, so as to reflect the influence of external factors on intracellular metabolism in real time. It can be used to study the metabolic rate of lactic acid in cells. |
GM-Probe-Adsensor-07 |
Adv-NES (nuclear export signal) indicator (mTurquoise reporter) |
NES(nuclear export signal), which is for export from the cell nucleus to the cytoplasm localization |
GM-Probe-Adsensor-08 |
Adv-Mito (mitochondrial)indicator (mTurquoise reporter) |
Mito (mitochondrial) localization |
GM-Probe-Adsensor-09 |
Adv-H2O2 biosensor (Laconic fluorescent protein) |
HyPer(Hydrogen peroxide sensor) is the first fully genetically encoded fluorescent sensor capable of detecting intracellular hydrogen peroxide (H2O2), one of the main ROS generated by cells. HyPer demonstrates submicromolar affinity to hydrogen peroxide and is insensitive to other oxidants tested, such as superoxide, oxidized glutathione, nitric oxide, and peroxinitrite. HyPer does not cause artifactual ROS generation and can be used for detection of fast changes of H2O2 concentration in different cell compartments under various physiological and pathological conditions. |
GM-Probe-Adsensor-10 |
Adv-Tubulin indicator (mTurquoise reporter) |
Tubulin cytoskeletal localization |
Note: mTurquoise is a monomeric cyan fluorescent protein with an excitation peak of 434nm and an emission peak of 474nm. It is an artificial derivative of the naturally occurring fluorescent protein encoded by the Aequorea victoria GFP gene (UniProtKB:P42212), derived by mutagenesis of SCFP3A, and is characterized by a long fluorescence lifetime (3.7ns) and a high quantum yield (0.84) (PMID:20081836). It contains the mutation T65S relative to SCFP3A (PMID:20081836), which itself contains the mutations F64L, S65T, Y66W, S72A, N146I, H148D, M153T, V163A, S175G and A206K relative to the wild-type GFP sequence (PMID:14990965).
