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Protocol of CRISPR /Cas9 mediated gene knockout in vitro and in vivo
Gene editing, also called genome editing, is a group of technologies to change the sequence of DNA in the genome. Several approaches to genome editing have been developed, including Zinc Finger, TALEN, and CRISPR/Cas9. Compared with the other gene editing tools, CRISPR/Cas9 system is faster, cheaper, more accurate, and more efficient, showing unprecedented advantages in gene therapy.
CRISPR/Cas9 premade products
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Cat No. | Products Name | Type of Crispr | Viral vector | Promoter | Fluorescent/Resistance | Tag | Order
|
|---|---|---|---|---|---|---|---|
Ad-CMV-spCas9 |
spCas9 |
Adenovirus |
CMV |
zsgreen |
FLAG |
||
Adv-CMV-spCas9-zsgreen |
spCas9 |
Adenovirus |
CMV |
EGFP |
FLAG |
||
Adv-CMV-spCas9-EGFP |
spCas9 |
Adenovirus |
CMV |
Null |
FLAG |
||
Adv-CMV-spCas9-mcherry |
spCas9 |
Adenovirus |
CMV |
mcherry |
FLAG |
||
spCas9 |
lentivirus |
CMV |
puromycin |
FLAG |
|||
spCas9 |
lentivirus |
CMV |
zsgreen |
FLAG |
|||
spCas9 |
lentivirus |
CMV |
EGFP |
FLAG |
|||
spCas9 |
lentivirus |
CMV |
mcherry |
FLAG |
|||
spCas9 |
lentivirus |
CBH |
puromycin |
FLAG |
|||
spCas9 |
lentivirus |
CBH |
zsgreen |
FLAG |
Genemedi has launched a comprehensive AAV packaging service combined with CRISPR/Cas9, the versatile genome-editing platform. The followings are some protocols of CRISPR /Cas9 mediated gene knockout in vitro and in vivo.
For AAV CRISPR/Cas9 service, please visit:
https://www.genemedi.com/i/aav-sacas9-packaging
For lentivirus CRISPR/Cas9 service, please visit:
https://www.genemedi.com/i/crispr-cas9-knockout-lentivirus-production-service
For Adenovirus CRISPR/Cas9 service, please visit:
https://www.genemedi.com/i/adenovirus-cas9-packaging
CRISPR/Cas9 AAV Production-User Manual
/pdf/Genemedi-AAV-Sa Cas9%20User%20Manual.pdf
Recombinant Adenovirus-CRISPR/Cas9 Knockout System-User Manual
https://www.genemedi.com/pdf/Genemedi-Adenovirus-crispr%20User%20Manual.pdf
Recombinant Lentivirus-CRISPR/Cas9 Knockout System-User Manual
/pdf/Genemedi-Lentivirus-crispr%20User%20Manual.pdf
View Crispr Knowledge Base>>
