Protocol of CRISPR /Cas9 mediated gene knockout in vitro and in vivo

Gene editing, also called genome editing, is a group of technologies to change the sequence of DNA in the genome. Several approaches to genome editing have been developed, including Zinc Finger, TALEN, and CRISPR/Cas9. Compared with the other gene editing tools, CRISPR/Cas9 system is faster, cheaper, more accurate, and more efficient, showing unprecedented advantages in gene therapy.

CRISPR/Cas9 premade products

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Cat No.
Products Name
Type of Crispr
Viral vector
Promoter
Fluorescent/Resistance
Tag
Order
Ad-CMV-spCas9
spCas9
Adenovirus
CMV
zsgreen
FLAG
Adv-CMV-spCas9-zsgreen
spCas9
Adenovirus
CMV
EGFP
FLAG
Adv-CMV-spCas9-EGFP
spCas9
Adenovirus
CMV
Null
FLAG
Adv-CMV-spCas9-mcherry
spCas9
Adenovirus
CMV
mcherry
FLAG
spCas9
lentivirus
CMV
puromycin
FLAG
spCas9
lentivirus
CMV
zsgreen
FLAG
spCas9
lentivirus
CMV
EGFP
FLAG
spCas9
lentivirus
CMV
mcherry
FLAG
spCas9
lentivirus
CBH
puromycin
FLAG
spCas9
lentivirus
CBH
zsgreen
FLAG
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Genemedi has launched a comprehensive AAV packaging service combined with CRISPR/Cas9, the versatile genome-editing platform. The followings are some protocols of CRISPR /Cas9 mediated gene knockout in vitro and in vivo. For AAV CRISPR/Cas9 service, please visit: https://www.genemedi.com/i/aav-sacas9-packaging For lentivirus CRISPR/Cas9 service, please visit: https://www.genemedi.com/i/crispr-cas9-knockout-lentivirus-production-service For Adenovirus CRISPR/Cas9 service, please visit: https://www.genemedi.com/i/adenovirus-cas9-packaging CRISPR/Cas9 AAV Production-User Manual /pdf/Genemedi-AAV-Sa Cas9%20User%20Manual.pdf Recombinant Adenovirus-CRISPR/Cas9 Knockout System-User Manual https://www.genemedi.com/pdf/Genemedi-Adenovirus-crispr%20User%20Manual.pdf Recombinant Lentivirus-CRISPR/Cas9 Knockout System-User Manual /pdf/Genemedi-Lentivirus-crispr%20User%20Manual.pdf View Crispr Knowledge Base>>