Cat:GMV-Crispr-AdV001
* GeneMedi provides NON-PROFIT PRICE to support Academic research. Please click inquiry for product quotation. → Inquiry
SKU GMV-Crispr-AdV001 Categories , ,

Description

Cat No.

GMV-Crispr-AdV001

Products Name

Ad-CMV-spCas9

Type of Crispr

spCas9

Viral vector

spCas9

Promoter

CMV

Fluorescent/Resistance

zsgreen

Tag

FLAG

Quantity/Unit

Vials

Form

Frozen form

Shipping and Storage Guidelines

Shipped by dry ice, stored at -80 ° C, effective for 1 year. Avoid repeatedly freezing and thawing

Titer

> 1*10^10 PFU/ml

description

Recombinant adenovirus (Adv), a replication-defective adenoviral vector system, is widely used for gene delivery in most cell types. The adenoviral vectors provided by Genemedi are based on human adenovirus type 5 (Ad5), which is replication-incompetent (-E1/-E3) and can’t be integrated into host genome, guaranteeing the security for subsequent operations. Adv packaging by Genemedi shows almost 100% gene delivery in most cell types in the recombinant protein expression system both in vivo and in vitro. Derived from the type II CRISPR-Cas bacterial adaptive immune system, Cas9, an RNA-guided endonuclease, has emerged as a versatile genome-editing platform. Several microbial species possess this system, among which, Streptococcus pyogenes Cas9 (SpCas9), is the most robust and widely used Cas9 to date, primarily recognizes NGG PAMs and is consequently restricted to sites that contain this motif. By combining Adv packaging system with Cas9 genome-editing platform, Genemedi has further developed Adv-Cas9 system to achieve gene knockout with high efficiency, which is one of the top advanced gene knockout technology in the world.

Advantages

1. Genome editing system of somatic tissues in postnatal animals. 2. Broad range of host. Adv has the ability to infect dividing, quiescent cells, stem cells, and primary cells, allowing genetic materials to be delivered to a highly diverse range of cell types and tissues. 3. High infection efficiency. As high as 100% gene delivery in most cell types, completely surpassing other viral vector tools and liposome transfection. 4. Without integration. No alteration to host genome. 5. High titer. Up to 1011 pfu/ml.

Quality control description

Our optimized custom adenoviral vector production and strict quality control systems provide customers a high titer of functional recombinant adenoviral vectors. Viral titers are determined by TCID50 method, which is the most accurate way to measure the titer of adenovirus.