Adenovirus protocol Download Adenovirus (AdV) is a member of the family Adenoviridae, whose name derives from their initial isolation from human adenoids in 1953 [1]. It is a medium-sized (90-100nm) and non-enveloped virus with an icosahedral nucleocapsid containing a double-stranded DNA genome. With a broad range of vertebrate hosts, 100 serotypes have been isolated, and […]
Category Archives: gene therapy
Lentivirus Protocol Download Lentivirus Production Protocol–Genemedi 1. Lentivirus plasmid construction The gene of interest is cloned into one of the LTR/MCS-containing lentivirus vectors to generate pLV-GOI. The purity and RNA contaminants of viral plasmid should be taken into consideration. 2. Lentivirus packaging The recombinant lentivirus viral plasmid pLV-GOI is co-transfected into the 293T with envelope […]
AAV protocol Download AAV Production Protocol–Genemedi 1. AAV plasmid construction The gene of interest is cloned into one of the ITR/MCS-containing AAV vectors to generate AAV-GOI. The purity and RNAse contaminants of AAV viral plasmid should be taken into consideration. 2. AAV packaging The recombinant AAV viral plasmid AAV-GOI is co-transfected into the AAV-293 cells […]
Lentivirus Protocol Download This protocol is for the stable cell line construction based on puromycin selection. Day 1: Seed target cells in 24-well plates. The number of seeding cells differs according to the cell proliferation rate.Day 2: Target cells should be approximately 50%-70% confluent. For polybrene accessible cells, mix the culture medium with proper concentrations of polybrene. […]
AAV protocol Download Adeno Associated Virus (AAV) For normal tissues or organs, such as heart, liver, kidney, breast, pancreas, ovary, brain, eye, skeleton muscle, adipose tissue, etc., Genemedi systematically organizes the corresponding optimal AAV serotype, gene delivery method and injection volume for mouse and rat tissue infection. Recommended AAV gene delivery methods for different organs is […]
CRISPR/Cas9 premade products Custom made CRISPR/Cas9 service Custom-made CRISPR/Cas9-gRNA lentivirusfor knockout cell line development Custom-made CRISPR/Cas9-gRNA adenovirusfor knockout cell line development Custom-made CRISPR/Cas9-gRNA AAVfor tissue-specific knockout in vivo CRISPR/Cas9 User Manual Crispr/cas9 mediated Gene knockout AAV Production CRISPR/Cas9Knockout System-User Manual Adenovirus CRISPR/Cas9Knockout System-User Manual Lentivirus-CRISPR/Cas9Knockout System-User Manual Introduction and principles of […]
Adenovirus Knowledge base What is Adenovirus vector? Adenovirus (AdV) is a member of the family Adenoviridae, whose name derives from their initial isolation from human adenoids in 1953 [1]. It is a medium-sized (90-100nm) and non-enveloped virus with an icosahedral nucleocapsid containing a double-stranded DNA genome. Read More Adenovirus Gene Therapy Review Adenovirus has been […]
Lentivirus Knowledge base What is Lentivirus Lentivirus (lente-, Latin for “slow”) is a genus of retroviruses, causing chronic and deadly diseases by long incubation periods in human or other mammalian species [1]. The virion is a medium-sized (80-100nm) and enveloped, slightly pleomorphic, spherical with an isometric nucleocapsid containing two copies of positive-sense ssRNA genome. Read […]
Adeno-associated virus (AAV) Knowledge base AAV serotypes and AAV Tissue-specific Tropism Over the past decades, numerous AAV serotypes have been identified with variable tropism. Read More Life Cycle of AAV Virus-From Infection To Regeneration AAV establishes latency by undergoing specifically integration into a genome site, termed as the adeno-associated virus integration site 1 (AAVS1), a […]
The Genemedi help scientists and researchers in gene therapy vector development. Besides different scales of AAV production, we can also offer novel AAV discovery services with AAV re-engineering capsids library and sub-library generation based on different strategies, customized AAV capsids screening and tissue-specific AAV tropism validation. 1. AAV engineering capsids library and sub-library generation 1) […]
