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AAV protocol Download

Adeno Associated Virus (AAV) For normal tissues or organs, such as heart, liver, kidney, breast, pancreas, ovary, brain, eye, skeleton muscle, adipose tissue, etc., Genemedi systematically organizes the corresponding optimal AAV serotype, gene delivery method and injection volume for mouse and rat tissue infection. Recommended AAV gene delivery methods for different organs is shown in Table5.

Table5. AAV gene delivery methods in specific organs.

Infection organRecommended serotypeInjection routeAnimalInjection volume (μl)
HeartAAV9Multiple points in situRat10-15/point, 3-5 points
Mouse10-15/point, 3-5 points
Tail veinRat250 (200g body weight)
Mouse100
LiverAAV8 or AAV9Tail veinRat200 (200g body weight)
Mouse100
Whole brainAAV-PHP.eB AAV-PHP.BTail veinRat200 (200g body weight)
Mouse100
Lateral ventricleAAV9StereotacticRat1-5
Mouse1-5
Brain tissueAAV9StereotacticRat2-3
Mouse1-2
FatAAV9Intraperitoneal injection for intraperitoneal fatRat300
Mouse150-200
in situ injection for subcutaneous fatRat10-15/point, 3-5 points
Mouse10-15/point, 3-5 points
Skeletal muscleAAV1 or AAV9In situ injectionRat10-15/point, 3-5 points
Mouse10-15/point, 3-5 points
EyesAAV2, AAV10 or AAV-DJvitreous chamber injectionRat3-5
Mouse1-3
subretinal space injectionRat1-2
Mouse1-2
LungAAV6Intratracheal injectionRat100 (200g weight)
Mouse50-75
KidneyAAV2 or AAV9Renal pelvis injectionRat10-15/point, 3-5 points
Mouse10-15/point, 3-5 points
IntestineAAV1 or AAV5EnemaRat200 (200g weight)
Mouse100

Detailed information of AAV in vivo animal injection protocol can be seen in AAV User Manual.


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