Adenovirus Knowledge base

What is Adenovirus vector?

Adenovirus (AdV) is a member of the family Adenoviridae, whose name derives from their initial isolation from human adenoids in 1953 [1]. It is a medium-sized (90-100nm) and non-enveloped virus with an icosahedral nucleocapsid containing a double-stranded DNA genome. 

Adenovirus Gene Therapy Review

Adenovirus has been proved as an excellent gene therapy vector. To date, more than 535 clinical trials have been carried out using adenovirus vectors for gene delivery [7], and promising gene therapy outcomes from recombinant adenovirus have been achieved from clinical trials for a great number of diseases (Table 1), especially for cancer treatment.

Advantages and Drawbacks of Adenovirus Vector-mediated Gene Transfer

Adenovirus has been developed into a preferred candidate for creating viral vectors for gene therapy due to various advantages.

Adenovirus Genome Structure (ITR, E1, E2A……) and adenovirus assembly

Adenovirus is a non-enveloped, 90-100 nm diameter virus presenting icosahedral symmetry (Figure1A). 

Life Cycle of Adenovirus-From Infection To Regeneration

For most serotypes, adenovirus infection is mediated by the high-affinity binding of the fiber-knob region to a receptor of target cell, named as the coxsackie-Ad receptor (CAR). 

Recombinant Adenovirus vector System (Adenovirus packaging System -AdMax system)

Since wild-type adenovirus is associated with a wide range of illnesses and enlists a variety of immune responses, so recombinant replication deficiency virus has been an attractive vector for gene therapy [20]. To date, there have been many different generations of adenovirus vectors, differing in the extent to which the genome from wild-type adenovirus is attenuated, among them, most studies involving adenovirus vectors utilize the simple E1-deleted, first-generation adenovirus vector. 

Adenovirus Vector Transduction – Adenovirus vector Gene Delivery in vitro and in vivo

For the reason that MOI varies in different cell lines, preliminary experiment is necessary to ensure a proper MOI of target cells before conducting formal experiments.