AAV1 vector system (AAV1 expression system, AAV1 packaging plasmid system )

GeneMedi’s AAV1 Vector System (AAV serotype 1 helper-free packaging plasmids system) is including AAV1 Rep-Cap plamid (AAV1-RC plasmid, or called AAV-RC1 plasmid), AAV helper plasmid and AAV expression vectors (overexpression or shRNA).

GeneMedi’s AAV expression vectors have been inserted with differernt expression cassettes, containing kinds of verified protomters and reporters including GFP, zsgreen, RFP, mcherry and luciferase. The GeneMedi’s AAV expression vectors have been proved very suitalble for unique gene overexpression or shRNA-mediated knock-down (also called RNAi (RNA interference ). You can also achieve gene knock-out(KO) or gene editing using our Crispr-cas9-gRNA AAV expression vector.

AAV1 Rep-Cap plamid supplies the AAV2 Rep(replication) proteins and the AAV1 capsid protein.

The tissue tropism of AAV1 vector has been validated in neuron(CNS), heart, muscle, retina and Pancreas, with potential applications in tissue-specific gene therapy.

AAV1 vector tissue tropism and gene transduction (serotype-specific AAV infection)

The tissue tropism of AAV1 vector has been validated in neuron(CNS), heart, muscle, retina and Pancreas, with potential applications in tissue-specific gene therapy.

Virus and titer: AAV1/AAV6/AAV8/AAV-Rh10/AAV-DJ/AAV9-GFP, 1×10¹² vg/ml
Cells: HEK-293T
MOI: MOI=1×10⁴
Determine assay: 36 hours post infection, immunofluorescence microscopy