AAV-DJ Rep-Cap plasmid (serotype DJ-specific AAV RC-DJ plasmids)

GeneMedi’s AAV-DJ Rep-Cap plasmid (serotype -DJ-specific AAV RC plasmid), also named AAV-DJ-RC plasmid, or AAV-RC-DJ plasmid, is part of AAV-DJ packaging system. AAV-DJ Rep-Cap plamid supplies the AAV-DJ Rep (replication) proteins and the AAV-DJ capsid protein.

You can produce AAV-DJ paticle in 293T cell line in high titer using GeneMedi’s AAV-DJ Rep-Cap plasmid with GeneMedi’s AAV expression vector (overexpression or shRNA) and AAV helper plasmid.

AAV-DJ Rep-Cap plamid supplies the AAV2 Rep(replication) proteins and the AAV-DJ capsid protein.

The tissue tropism of AAV-DJ vector has been validated in retina, lung, liver and kidney, with potential applications in tissue-specific gene therapy.

AAV-DJ vector tissue tropism and gene transduction (serotype-specific AAV infection)

The tissue tropism of AAV-DJ vector has been validated in retina, lung, liver and kidney, with potential applications in tissue-specific gene therapy.

Virus and titer: AAV-DJ-GFP, AAV9-GFP, 1×10¹² vg/ml
Animal: mouse, C57, 2 months
Infection site: Kidney
Gene delivery method: Multiple sites injection in kidney 10μl/site, 6 sites in total
Determine assay: 3 weeks post infection, frozen section, immunofluorescence microscopy

Virus and titer: AAV1/AAV6/AAV8/AAV-Rh10/AAV-DJ/AAV9-GFP, 1×10¹² vg/ml
Cells: HEK-293T
MOI: MOI=1×10⁴
Determine assay: 36 hours post infection, immunofluorescence microscopy