AAV8 vector system (AAV8 expression system, AAV8 packaging plasmid system )

GeneMedi’s AAV8 Vector System (AAV serotype 8 helper-free packaging plasmids system) is including AAV8 Rep-Cap plamid (AAV8-RC plasmid, or called AAV-RC8 plasmid), AAV helper plasmid and AAV expression vectors (overexpression or shRNA).

GeneMedi’s AAV expression vectors have been inserted with differernt expression cassettes,containing kinds of verified protomters and reporters including GFP, zsgreen, RFP, mcherry and luciferase. The GeneMedi’s AAV expression vectors have been proved very suitalble for unique gene overexpression or shRNA-mediated knock-down (also called RNAi (RNA interference ). You can also achieve gene knock-out(KO) or gene editing using our Crispr-cas9-gRNA AAV expression vector.

AAV8 Rep-Cap plamid supplies the AAV2 Rep(replication) proteins and the AAV8 capsid protein.

The tissue tropism of AAV8 vector has been validated in retina, liver, pancreas and muscle, with potential applications in tissue-specific gene therapy.

AAV8 vector tissue tropism and gene transduction (serotype-specific AAV infection)

The tissue tropism of AAV8 vector has been validated in retina, liver, pancreas and muscle, with potential applications in tissue-specific gene therapy.

Virus and titer: AAV1/AAV6/AAV8/AAV-Rh10/AAV-DJ/AAV9-GFP, 1×10¹² vg/ml
Cells: HEK-293T
MOI: MOI=1×10⁴
Determine assay: 36 hours post infection, immunofluorescence microscopy