human SHH AAV plasmid

Cat:pGMAAV000001
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Description

Catalog ID

pGMAAV000001

Gene Name

SHH

Product Name

human SHH AAV plasmid

Accession Number

NM_000193.3

Gene ID

6469

Species

human

Product Type

AAV plasmid

Insert Length

1389

Gene Alias

HHG1;HLP3;HPE3;MCOPCB5;ShhNC;SMMCI;TPT;TPTPS

Fluorescent Label

GFP

Fusion Tag

no tag

Promoter

CMV

Resistance

Amplicin

gene description

This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly. [provided by RefSeq, Jul 2008]

Maker

Regulation

overexpression