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Lentivirus-GFP-LC3
Cat:GM-LVPAU02
* GeneMedi provides NON-PROFIT PRICE to support Academic research. Please click inquiry for product quotation. → Inquiry
Description
Catalog No.
GM-LVPAU02
Product Name
Lentivirus-GFP-LC3
Production Scale
AAV packaging Serotype:
Final Deliverables
Lead Time(workday)
Quantity/Unit
Vials
Form
Frozen form
Shipping and Storage Guidelines
Shipped by dry ice, stored at -80 ℃, effective for 1 year. Avoid repeatedly freezing and thawing
Advantages
1. Pre-packaged, ready-to-use, fluorescently-tagged with monomeric GFP & RFP.
2. Higher efficiency transfection as compared to traditional chemical-based and other non-viral-based transfection methods. Ability to transfect dividing, non-dividing, and difficult-to-transfect cell types, such as primary cells or stem cells.
3. Non-disruptive towards cellular function.
4. Reveals changing cellular conditions in real time. Enables visualization under different cell/disease states in live cell and in vitro analysis.
Applications and Figures
Quality control description
Our optimized production of lentiviral vector and strict quality control systems supply customers with a high titer of functional recombinant lentiviral vectors. Two methods are employed to determine viral titers: physical titer (VP/mL) and functional titer (TU/mL). Physical titer is calculated by the level of protein, such as p24, or viral nucleic acid. The functional titer, a calculation of the active virus that can infect cells, is much less than the physical titer (100-1000 fold lower). The method we adopted is functional titer, which is an accurate solution for testing virus accurate activity and MOI. The physical titer can only reflect the number of virus particles, but not reflect the true viral activity, which will cause a large error in subsequent infection experiments.
Technical Documents
1. For further information about AAV administration and transduction results, please see the 
AAV(adeno associated virus) User Manual.
2. For more information about how to use in vivo Autophagy Flux Detection with AAV vectors, please refer to the AAV-LC3 Autophagy Flux Detection Manual.
AAV(adeno associated virus) User Manual.
2. For more information about how to use in vivo Autophagy Flux Detection with AAV vectors, please refer to the AAV-LC3 Autophagy Flux Detection Manual. Frequently Asked Questions(FAQs)
Reference
1.Wu, J. et al. MicroRNA-30 family members regulate calcium/calcineurin signaling in podocytes. Journal of Clinical Investigation 125, 4091-4106 (2015).
2.Li, F., Li, S. & Cheng, T. TGF-β1 Promotes Osteosarcoma Cell Migration and Invasion Through the miR- 143-Versican Pathway. Cellular Physiology and Biochemistry 34, 2169-2179 (2014).
3.Liu, Z. et al. miR-451a Inhibited Cell Proliferation and Enhanced Tamoxifen Sensitive in Breast Cancer via Macrophage Migration Inhibitory Factor. BioMed Research International 2015, 207684-207684 (2015).
4.Si, L. et al. Smad4 mediated BMP2 signal is essential for the regulation of GATA4 and Nkx2.5 by affecting the histone H3 acetylation in H9c2 cells. Biochemical and Biophysical Research Communications 450, 81-86 (2014).
5.Han, H., Yang, S., Lin, S. G., Xu, C. S. & Han, Z. Effects and mechanism of downregulation of COX‑2 expression by RNA interference on proliferation and apoptosis of human breast cancer MCF‑7 cells. Molecular Medicine Reports 10, 3092-3098 (2014).
6.Zhang, G., Liu, Z., Cui, G., Wang, X. & Yang, Z. MicroRNA-486-5p targeting PIM-1 suppresses cell proliferation in breast cancer cells. Tumor Biology 35, 11137-11145 (2014).
7.Li, G. et al. CYC1 silencing sensitizes osteosarcoma cells to TRAIL-induced apoptosis. Cellular Physiology and Biochemistry 34, 2070-2080 (2014).
8.Mao, J., Lv, Z. & Zhuang, Y. MicroRNA-23a is involved in tumor necrosis factor-α induced apoptosis in mesenchymal stem cells and myocardial infarction. Experimental and Molecular Pathology 97, 23-30 (2014).
9.Liu, X. et al. Role of human pulmonary fibroblast-derived MCP-1 in cell activation and migration in experimental silicosis. Toxicology and Applied Pharmacology 288, 152-160 (2015).
10.Guan, G. et al. CXCR4-targeted near-infrared imaging allows detection of orthotopic and metastatic human osteosarcoma in a mouse model. Scientific Reports 5, 15244-15244 (2015).
11.Zhang, Y. et al. Role of high-mobility group box 1 in methamphetamine-induced activation and migration of astrocytes. Journal of Neuroinflammation 12, 156-156 (2015).
12.Zhu, T. et al. The Role of MCPIP1 in Ischemia/Reperfusion Injury-Induced HUVEC Migration and Apoptosis. Cellular Physiology and Biochemistry 37, 577-591 (2015).
13.Qian, M. et al. P50-associated COX-2 extragenic RNA (PACER) overexpression promotes proliferation and metastasis of osteosarcoma cells by activating COX-2 gene. Tumor Biology 37, 3879-3886 (2016).
14.Wu, N., Song, Y., Pang, L. & Chen, Z. CRCT1 regulated by microRNA-520 g inhibits proliferation and induces apoptosis in esophageal squamous cell cancer. Tumor Biology 37, 8271-8279 (2016).
15.Wang, Y. et al. Overexpression of Hiwi Inhibits the Growth and Migration of Chronic Myeloid Leukemia Cells. Cell Biochemistry and Biophysics 73, 117-124 (2015).
16.Niu, L. et al. RNF43 Inhibits Cancer Cell Proliferation and Could be a Potential Prognostic Factor for Human Gastric Carcinoma. Cellular Physiology and Biochemistry 36, 1835-1846 (2015).
17.Zhang, H. et al. ZC3H12D attenuated inflammation responses by reducing mRNA stability of proinflammatory genes. Molecular Immunology 67, 206-212 (2015).
18.Deng, X. et al. MiR-146b-5p Promotes Metastasis and Induces Epithelial-Mesenchymal Transition in Thyroid Cancer by Targeting ZNRF3. Cellular Physiology and Biochemistry 35, 71-82 (2015).
19.Zhang, B. et al. HSF1 Relieves Amyloid-β-Induced Cardiomyocytes Apoptosis. Cell Biochemistry and Biophysics 72, 579-587 (2015).
20.Hu, Q. et al. Periostin Mediates TGF-β-Induced Epithelial Mesenchymal Transition in Prostate Cancer Cells. Cellular Physiology and Biochemistry 36, 799-809 (2015).
21.Yang, Z. et al. CD49f Acts as an Inflammation Sensor to Regulate Differentiation, Adhesion, and Migration of Human Mesenchymal Stem Cells. Stem Cells 33, 2798-2810 (2015).
22.Wang, X. et al. MCPIP1 Regulates Alveolar Macrophage Apoptosis and Pulmonary Fibroblast Activation After in vitro Exposure to Silica. Toxicological Sciences 151, 126-138 (2016).
