Diagnostic anti-gp50 antibodies pairs and antigens for animal health (animal infectious disease ) testing in ELISA test,competitive ELISA test, blocking ELISA test, Lateral flow immunoassay (LFIA), colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA), turbidimetric inhibition immuno assay (TINIA), and immunonephelometry

Catalog Number:GMP-AD-Pig-15
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Product Details

GP50, a Taenia solium protein diagnostic for cysticercosis has been cloned, sequenced, and characterized. GP50 is one diagnostic component of the lentil lectin purified glycoprotein (LLGP) antigens that have been used for antibody-based diagnosis of cysticercosis in a Western blot assay for nearly 15 years. GP50 is a glycosylated and GPI-anchored membrane protein. The native protein migrates at 50kDa, but the predicted molecular weight of the mature protein is 28.9. Antigenically active recombinant GP50 has been expressed in a baculovirus expression system. The antigenic activity of both the native and recombinant proteins is dependent upon the correct formation of disulfide bonds. GP50, purified from cysticerci, has two homologs expressed in the adult worm, TSES33 and TSES38. Both are diagnostic for taeniasis. In spite of the amino acid similarities between GP50 and the TSES proteins, each appears to be a stage-specific antigen. A preliminary evaluation of recombinant GP50 in a Western blot assay showed 100% specificity for cysticercosis and 90% sensitivity for cysticercosis positive serum samples reactive with the GP50 component of LLGPGenemedi produces core animal health diagnostic ingredients-validated antibodies pairs Mouse anti-gp50 monoclonal antibodies and gp50 antigens for rapid test kit of animal infectious disease with gp50 to evaluate the animal health of Pig.The paired antibodies are both monoclonal antibody(mab).
All the antibodies and antiges of animal disease test are suitable for in functional ELISA, and other immunoassays in dignostics.The antibody can act as a capture antibody and detection antibody. Antigens are validated as positive control materials.

Product Description

Cat No.

GMP-AD-Pig-15Ab

Antigens

gp50

Antibody

Mouse anti-gp50 monoclonal antibodies

Resource (expression host)

hybridoma

Specics/Isotypes

Mouse IgG

Bioactivity validation

Antibody Binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays in gp50 level test and Pig-diagnositcs.

Antigen description

Purity

Purity: ≥95% (SDS-PAGE)

Application

Paired antibody immunoassay validation in sandwich Elisa, Lateral flow immunoassay (LFIA), and other immunoassays;

Formulation

Lyophilized from sterile PBS, PH 7.4

Storage

Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.

GMP-AD-Pig-15Ag

Antigens

gp50

Resource (expression host)

E.coli

Specics/Isotypes

gp50

Bioactivity validation

Antibody Binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays in gp50 level test and Pig-diagnositcs.

Tag

His

Antigen description

GP50, a Taenia solium protein diagnostic for cysticercosis has been cloned, sequenced, and characterized. GP50 is one diagnostic component of the lentil lectin purified glycoprotein (LLGP) antigens that have been used for antibody-based diagnosis of cysticercosis in a Western blot assay for nearly 15 years. GP50 is a glycosylated and GPI-anchored membrane protein. The native protein migrates at 50kDa, but the predicted molecular weight of the mature protein is 28.9. Antigenically active recombinant GP50 has been expressed in a baculovirus expression system. The antigenic activity of both the native and recombinant proteins is dependent upon the correct formation of disulfide bonds. GP50, purified from cysticerci, has two homologs expressed in the adult worm, TSES33 and TSES38. Both are diagnostic for taeniasis. In spite of the amino acid similarities between GP50 and the TSES proteins, each appears to be a stage-specific antigen. A preliminary evaluation of recombinant GP50 in a Western blot assay showed 100% specificity for cysticercosis and 90% sensitivity for cysticercosis positive serum samples reactive with the GP50 component of LLGP

Purity

Purity: ≥95% (SDS-PAGE)

Application

Paired antibody immunoassay validation in sandwich Elisa, Lateral flow immunoassay (LFIA), and other immunoassays;

Formulation

Lyophilized from sterile PBS, PH 7.4

Storage

Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Validated animal health diagnostic antibodies pairs and antigens for animal infectious diseases diagnostic testing in ELISA, Lateral flow immunoassay (LFIA) and other immunoassays

Livestock, poultry, and aquaculture are among the fastest growing and expanding agriculture sectors to fulfill the need of the growing population of humans. However, the growth in this sector is under the continuous increasing threats of infectious diseases worldwide. This menace is further aggravated by globalization in animal trade for various purposes. The sudden entry of an infectious disease in a new country or geographical location could lead to delayed diagnosis and rapid spread into the susceptible animal population. Hence, Animal diagnostics are critical for animal health, identifying health issues before they are otherwise able to be detected and supporting faster diagnosis and treatment planning. Diagnosis is an essential part of disease management and prevention. The importance of animal disease diagnostics laboratories is not a very recognized area of animal production but they are essential to animal health. It is important for not only animal producers, but also consumers to be aware of this resource. The application of innovative diagnostic technologies for the detection of animal pathogens at an early stage is essential in restricting the economic loss incurred due to emerging infectious animal diseases. The desirable characteristics of such diagnostic methods are easy to use, cost-effective, highly sensitive, and specific, coupled with the high-throughput detection capabilities. Genemedi provides diagnostic antibodies and antigens for the in vitro diagnosis of diseases from the pet, Cat/Feline, Dog/Canine, Rabbit, Bovines/Cattle, Ovines/Sheep, Caprine/Goat, Equine/Horse, Swine/Porcine/Pig, Avian/bird/poultry, Fish and so on.