Immunofluorescence staining (IF) - strategies used in diagnosis of animal infectious disease for animal health
The immunofluorescence staining is to detect viral antigen using virus-specific monoclonal or polyclonal antibodies fluorescent staining of viral antigens is visualized under a fluorescent microscope.
In the recent years, importance of animal disease and their public health effects have been well recognized worldwide. Animal disease, more significantly, zoonotic disease cause human mortality and morbidity, and also affect livestock’s production, decrease availability of food and create barriers for international trade. Rapid diagnosis is critical for the implementation of efficient control strategies against animal disease and zoonotic disease. Understanding animal disease infection dynamics and collecting appropriate specimens at the appropriate time window are also important to obtain reliable diagnostic results. A number of virological and serological methods have been developed and used for animal disease diagnostic testing. RT-PCR is the method of common choice for the detection of animal disease; IHC combined with hematoxylin and eosin staining has also been commonly used to examine histopathological lesions caused by animal disease. Success rate of virus isolation in cell cultures has been low. Serological assays can provide information about previous exposure to animal disease and also determine antibody responses to infection or vaccination when vaccines are available. Rolling out serological test would be an effective strategy to determine the percentage of the population that is immune and have shown no symptoms for the animal disease. Thereby, determining the exact magnitude of the outbreak and enabling governments to assess containment strategies to slow down the spread. The major drawbacks with these immunoassays are their accuracy and sensitivity of the test results. Therefore, there needs to be extensive research and testing done to develop new cost-effective methods to quickly and easily determine animal disease infection. Whereas, any such emerging approach must be carefully evaluated for its efficiency, accuracy, and linear range. The FDA approval and evaluation of each diagnostic technique is necessary before it can be used in practice.
Full product list: Ruminants, Pet, Swine, Equine, Avian, Fish, Multiple Species
Validated animal health diagnostic antibodies pairs and antigens for animal infectious diseases diagnostic testing in ELISA, Lateral flow immunoassay (LFIA) and other immunoassays.
GeneMedi offers paired antibodies and antigens for Animal Health Diagnostic testing including most of the infectious disease in different animals:Avian(birds), fish, pets(cat, dog, rabbit), pig, ruminants(cow, goat, sheep, ox, cattle, bull) and so on(please see below).
All our animal health diagnostic antibodies and antigens for antimals infectious diseases test are suitable for in functional sandiwich ELISA, and other immunoassays in diagnostics. The antibodies can act as a capture antibody and detection antibody. The antigens can be used for antibodies rapid test of infectious disease.
1. Ryu, S., et al., One Health Perspectives on Emerging Public Health Threats. J Prev Med Public Health, 2017. 50(6): p. 411-414.
2. World Health Organization (WHO). Zoonoses. Accessed October 3, 2018.; Available from: http://www.who.int/zoonoses.
3. Leslie MJ, M.J. Surveillance for zoonotic diseases. BLUKO97- Mikanatha 2007; Available from: http://courses.washington.edu/zepi526/Papers08/Rabies%20chapter.pdf.
4. Available from: https://www.doctorsgate.com/en/what-are-the-current-diagnostic-tests-for-covid-19/.
5. Anylab. Available from: www.zetbio.com.
6. Li, H., et al., A new and rapid approach for detecting COVID-19 based on S1 protein fragments. Clin Transl Med, 2020. 10(2): p. e90.
7. Khanna, M., et al., Evaluation of influenza virus detection by direct enzyme immunoassay (EIA) and conventional methods in asthmatic patients. J Commun Dis, 2001. 33(3): p. 163-9.
8. Waner, J.L., et al., Comparison of Directigen FLU-A with viral isolation and direct immunofluorescence for the rapid detection and identification of influenza A virus. J Clin Microbiol, 1991. 29(3): p. 479-82.
9. Cameron, J.D., A.P. Skubitz, and L.T. Furcht, Type IV collagen and corneal epithelial adhesion and migration. Effects of type IV collagen fragments and synthetic peptides on rabbit corneal epithelial cell adhesion and migration in vitro. Invest Ophthalmol Vis Sci, 1991. 32(10): p. 2766-73.
10. Kim, M.H., S.Y. Kang, and W.I. Lee, Evaluation of a new rapid test kit to detect hepatitis C virus infection. J Virol Methods, 2013. 193(2): p. 379-82.
11. Niu, X., et al., [Establishment of the evaluation reference system for domestic anti-hepatitis C virus diagnostic enzyme immunoassay kits]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, 2013. 29(7): p. 761-4.
12. Filice, G., et al., Sensitivity and specificity of anti-HIV ELISA employing recombinant (p24, p66, gp120) and synthetic (gp41) viral antigenic peptides. Microbiologica, 1991. 14(3): p. 185-94.
13. de Boer, G.F., W. Back, and A.D. Osterhaus, An ELISA for detection of antibodies against influenza A nucleoprotein in humans and various animal species. Arch Virol, 1990. 115(1-2): p. 47-61.
14. Cuzzubbo, A.J., et al., Comparison of PanBio dengue duo enzyme-linked immunosorbent assay (ELISA) and MRL dengue fever virus immunoglobulin M capture ELISA for diagnosis of dengue virus infections in Southeast Asia. Clin Diagn Lab Immunol, 1999. 6(5): p. 705-12.
15. Maria-C-Jimenez-Martinez. Available from:
16. sciencephoto. Available from: https://www.sciencephoto.com/media/90184/view.
17. Uyeki, T.M., et al., Clinical Practice Guidelines by the Infectious Diseases Society of America: 2018 Update on Diagnosis, Treatment, Chemoprophylaxis, and Institutional Outbreak Management of Seasonal Influenzaa. Clin Infect Dis, 2019. 68(6): p. e1-e47.
18. Goldsmith, C. Hantavirus Life Cycle and Infection Process. Available from: https://www.hantasite.com/2017/03/hantavirus-life-cycle-and-infection.html.
19. METHODS, M. 2013; 3:207
20. biotech, G.; Available from: https://www.globalbiotechinsights.com/articles/20247/the-worldwide-test-for-covid-19.