Crispr Knowledge base

CRISPR/Cas9 premade products

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Type of Crispr
Viral vector
Promoter
Fluorescent/Resistance
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spCas9
lentivirus
CBH
EGFP
FLAG
spCas9
lentivirus
CBH
mcherry
FLAG
AAV-CMV-saCas9
saCas9
AAV
CMV
Null
HA
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Custom made CRISPR/Cas9 service

Custom-made CRISPR/Cas9-gRNA lentivirus
for knockout cell line development

Custom-made CRISPR/Cas9-gRNA adenovirus
for knockout cell line development

Custom-made CRISPR/Cas9-gRNA AAV
for tissue-specific knockout in vivo

Introduction and principles of CRISPR/Cas9/Cas12/Cas13/Cpf1 gene editing system

CRISPR (clustered regularly interspaced short palindromic repeats) is a family of DNA sequences derived from viruses that have previously infected the prokaryotes and has the ability to recognize invasive homologous DNA sequences from similar viruses.

What is CRISPR/Cas9 delivery system

CRISPR/Cas9 system can be delivered in vitro by the non-viral reagents (such as polymer, liposome, nano-particle, electroporation) or viral vectors (such as lentivirus, adenovirus and AAV) and in vivo by adenovirus or AAV vectors.

CRISPR/Cas9 system – gRNA design and validation -principle and protocol

The PAM sequence that SpCas9 recognizes is NGG (AGG, TGG, CGG, GGG), while SaCas9 recognizes NNGRRT (NNGAAT, NNGAGT, NNGGAT, NNGGGT) or NNGRRN. For sgRNA, the length is about 21 or 22 nucleotides.