Gene Transfection, in layman’s words, mainly refers to the increase and decline of gene expression (i.e. gain of function OR loss of function). A classic animal model of gene intervention–genetically engineered mice.Genetically engineered mice included transgenic mice (TG mouse), Knockout mice (KO mouse), and typed mice (KI mouse). In the introduction of CRE-LOXP system, the […]
In vitro (Latin: in glass) studies in experimental biology are those that are conducted using components of an organism that have been isolated from their usual biological surroundings in order to permit a more detailed or more convenient analysis than can be done with whole organisms. Colloquially, these experiments are commonly called “test tube experiments”. In […]
Transfection in vivo Transfection in vitro can be mediated by the non-viral reagents (such as liposome, polymer, nano-particle) or viral vectors (such as lentiviruses, adenovirus and AAV). Non-viral methods Formulations of liposome, polymer and nano-particle have been optimized for in vivo delivery, especially with the use of transfection enhancer and secondary injection. What’s more, the […]
Transfection protocol Download Transfection in vitro Transfection in vitro can be mediated by the non-viral reagents (such as polymer, liposome, nano-particle) or viral vectors (such as lentivirus, adenovirus and AAV). Polymer-based transfection Polymer transfection is a technique used to deliver DNA or RNA into cells with biodegradable cationic polymer [6], such as DEAE-dextran or polyethylenimine […]
Transfection protocol Download In general, transfection is the process of delivering nucleic acids, such as DNA and RNA into eukaryotic cells, resulting in the expression or production of proteins or down-regulation of the targeted protein. Although not very common, protein transfection is also used to promote the rapid expression of target protein (such as Cas9 […]
CRISPR/Cas9 protocol Download Gene editing, also called genome editing, is a group of technologies to change the sequence of DNA in the genome. Several approaches to genome editing have been developed, including Zinc Finger, TALEN, and CRISPR/Cas9. Compared with the other gene editing tools, CRISPR/Cas9 system is faster, cheaper, more accurate, and more efficient, showing […]
Small interfering RNA (siRNA) transfection Small interfering RNA (siRNA) contains 21-25 nucleotides, specific for target RNA. Once entry into cells, siRNAs can be recruited into a multi-protein complex, known as RNA induced silencing complex (RISC), which interacts with the target RNA to mediate mRNA degradation, thus knock-down or suppress the expression of target gene [45]. […]
