AAV-DJ vector system (AAV-DJ expression system, AAV-DJ packaging plasmid system)

GeneMedi’s AAV-DJ Vector System (AAV serotype-DJ helper-free packaging plasmids system) is including AAV-DJ Rep-Cap plamid (AAV-DJ-RC plasmid, or called AAV-RC-DJ plasmid), AAV helper plasmid and AAV expression vectors (overexpression or shRNA).

GeneMedi’s AAV expression vectors have been inserted with differernt expression cassettes, containing kinds of verified protomters and reporters including GFP, zsgreen, RFP, mcherry and luciferase. The GeneMedi’s AAV expression vectors have been proved very suitalble for unique gene overexpression or shRNA-mediated knock-down (also called RNAi (RNA interference). You can also achieve gene knock-out(KO) or gene editing using our Crispr-cas-DJ-gRNA AAV expression vector.

AAV-DJ Rep-Cap plamid supplies the AAV2 Rep(replication) proteins and the AAV-DJ capsid protein.

The tissue tropism of AAV-DJ vector has been validated in retina, lung, liver and kidney, with potential applications in tissue-specific gene therapy.

AAV-DJ vector tissue tropism and gene transduction (serotype-specific AAV infection)

The tissue tropism of AAV-DJ vector has been validated in retina, lung, liver and kidney, with potential applications in tissue-specific gene therapy.

Virus and titer: AAV-DJ-GFP, AAV9-GFP, 1×10¹² vg/ml
Animal: mouse, C57, 2 months
Infection site: Kidney
Gene delivery method: Multiple sites injection in kidney 10μl/site, 6 sites in total
Determine assay: 3 weeks post infection, frozen section, immunofluorescence microscopy

Virus and titer: AAV1/AAV6/AAV8/AAV-Rh10/AAV-DJ/AAV9-GFP, 1×10¹² vg/ml
Cells: HEK-293T
MOI: MOI=1×10⁴
Determine assay: 36 hours post infection, immunofluorescence microscopy