AAV-DIO in neuroscience |-GeneMedi

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Product list: Cre/loxP tools in AAV vector(AAV-Cre), adenoviral vector(Ad-Cre) and lentiviral vector(Lv-Cre)

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AAV9-GFAP-Cre-EGFP
AAV GeneMedi offer multiple serotypes of AAV-Cre loxP system for your option: · AAV1-Cre · AAV2-Cre · AAV2 variant (Y444F)-Cre · AAV2 variant (Y272F, Y444F, Y500F, Y730F)-Cre · AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F)-Cre · AAV2 variant(AAV2.7m8)-Cre · AAV5-Cre · AAV6-Cre · AAV8-Cre · AAV8-1m-Cre · AAV8-2m-Cre · AAV8 variant (Y733F, Y447F, Y275)-Cre · AAV9-Cre · AAV-Rh.10-Cre · AAV-DJ-Cre · AAV-DJ/8-Cre · AAV-Retro (Retrograde)-Cre · AAV9-PHP.B-Cre · AAV9-PHP.A-Cre · AAV9-PHP.eB-Cre · AAV9-PHP.S-Cre
GFAP
Neuro
Astrocyte specific promoter
AAV9-GFAP-Cre
AAV GeneMedi offer multiple serotypes of AAV-Cre loxP system for your option: · AAV1-Cre · AAV2-Cre · AAV2 variant (Y444F)-Cre · AAV2 variant (Y272F, Y444F, Y500F, Y730F)-Cre · AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F)-Cre · AAV2 variant(AAV2.7m8)-Cre · AAV5-Cre · AAV6-Cre · AAV8-Cre · AAV8-1m-Cre · AAV8-2m-Cre · AAV8 variant (Y733F, Y447F, Y275)-Cre · AAV9-Cre · AAV-Rh.10-Cre · AAV-DJ-Cre · AAV-DJ/8-Cre · AAV-Retro (Retrograde)-Cre · AAV9-PHP.B-Cre · AAV9-PHP.A-Cre · AAV9-PHP.eB-Cre · AAV9-PHP.S-Cre
GFAP
Neuro
Astrocyte specific promoter
AAV9-cTNT-Cre
AAV GeneMedi offer multiple serotypes of AAV-Cre loxP system for your option: · AAV1-Cre · AAV2-Cre · AAV2 variant (Y444F)-Cre · AAV2 variant (Y272F, Y444F, Y500F, Y730F)-Cre · AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F)-Cre · AAV2 variant(AAV2.7m8)-Cre · AAV5-Cre · AAV6-Cre · AAV8-Cre · AAV8-1m-Cre · AAV8-2m-Cre · AAV8 variant (Y733F, Y447F, Y275)-Cre · AAV9-Cre · AAV-Rh.10-Cre · AAV-DJ-Cre · AAV-DJ/8-Cre · AAV-Retro (Retrograde)-Cre · AAV9-PHP.B-Cre · AAV9-PHP.A-Cre · AAV9-PHP.eB-Cre · AAV9-PHP.S-Cre
cTnT
heart
Cardiomyocyte specific promoter
AAV9-cTNT-Cre-EGFP
AAV GeneMedi offer multiple serotypes of AAV-Cre loxP system for your option: · AAV1-Cre · AAV2-Cre · AAV2 variant (Y444F)-Cre · AAV2 variant (Y272F, Y444F, Y500F, Y730F)-Cre · AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F)-Cre · AAV2 variant(AAV2.7m8)-Cre · AAV5-Cre · AAV6-Cre · AAV8-Cre · AAV8-1m-Cre · AAV8-2m-Cre · AAV8 variant (Y733F, Y447F, Y275)-Cre · AAV9-Cre · AAV-Rh.10-Cre · AAV-DJ-Cre · AAV-DJ/8-Cre · AAV-Retro (Retrograde)-Cre · AAV9-PHP.B-Cre · AAV9-PHP.A-Cre · AAV9-PHP.eB-Cre · AAV9-PHP.S-Cre
cTnT
heart
Cardiomyocyte specific promoter
AAV9-TBG-Cre
AAV GeneMedi offer multiple serotypes of AAV-Cre loxP system for your option: · AAV1-Cre · AAV2-Cre · AAV2 variant (Y444F)-Cre · AAV2 variant (Y272F, Y444F, Y500F, Y730F)-Cre · AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F)-Cre · AAV2 variant(AAV2.7m8)-Cre · AAV5-Cre · AAV6-Cre · AAV8-Cre · AAV8-1m-Cre · AAV8-2m-Cre · AAV8 variant (Y733F, Y447F, Y275)-Cre · AAV9-Cre · AAV-Rh.10-Cre · AAV-DJ-Cre · AAV-DJ/8-Cre · AAV-Retro (Retrograde)-Cre · AAV9-PHP.B-Cre · AAV9-PHP.A-Cre · AAV9-PHP.eB-Cre · AAV9-PHP.S-Cre
TBG
liver
Hepatic specific promoter
AAV9-TBG-Cre-ZsGreen
AAV GeneMedi offer multiple serotypes of AAV-Cre loxP system for your option: · AAV1-Cre · AAV2-Cre · AAV2 variant (Y444F)-Cre · AAV2 variant (Y272F, Y444F, Y500F, Y730F)-Cre · AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F)-Cre · AAV2 variant(AAV2.7m8)-Cre · AAV5-Cre · AAV6-Cre · AAV8-Cre · AAV8-1m-Cre · AAV8-2m-Cre · AAV8 variant (Y733F, Y447F, Y275)-Cre · AAV9-Cre · AAV-Rh.10-Cre · AAV-DJ-Cre · AAV-DJ/8-Cre · AAV-Retro (Retrograde)-Cre · AAV9-PHP.B-Cre · AAV9-PHP.A-Cre · AAV9-PHP.eB-Cre · AAV9-PHP.S-Cre
TBG
liver
Hepatic specific promoter
AAV9-TIE-Cre
AAV GeneMedi offer multiple serotypes of AAV-Cre loxP system for your option: · AAV1-Cre · AAV2-Cre · AAV2 variant (Y444F)-Cre · AAV2 variant (Y272F, Y444F, Y500F, Y730F)-Cre · AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F)-Cre · AAV2 variant(AAV2.7m8)-Cre · AAV5-Cre · AAV6-Cre · AAV8-Cre · AAV8-1m-Cre · AAV8-2m-Cre · AAV8 variant (Y733F, Y447F, Y275)-Cre · AAV9-Cre · AAV-Rh.10-Cre · AAV-DJ-Cre · AAV-DJ/8-Cre · AAV-Retro (Retrograde)-Cre · AAV9-PHP.B-Cre · AAV9-PHP.A-Cre · AAV9-PHP.eB-Cre · AAV9-PHP.S-Cre
TIE
endothelial
Endothelial-specific promoter
AAV9-cd68-Cre
AAV GeneMedi offer multiple serotypes of AAV-Cre loxP system for your option: · AAV1-Cre · AAV2-Cre · AAV2 variant (Y444F)-Cre · AAV2 variant (Y272F, Y444F, Y500F, Y730F)-Cre · AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F)-Cre · AAV2 variant(AAV2.7m8)-Cre · AAV5-Cre · AAV6-Cre · AAV8-Cre · AAV8-1m-Cre · AAV8-2m-Cre · AAV8 variant (Y733F, Y447F, Y275)-Cre · AAV9-Cre · AAV-Rh.10-Cre · AAV-DJ-Cre · AAV-DJ/8-Cre · AAV-Retro (Retrograde)-Cre · AAV9-PHP.B-Cre · AAV9-PHP.A-Cre · AAV9-PHP.eB-Cre · AAV9-PHP.S-Cre
cd68
Neuro
Microglia-specific promoter
AAV9-c-fos-Cre
AAV GeneMedi offer multiple serotypes of AAV-Cre loxP system for your option: · AAV1-Cre · AAV2-Cre · AAV2 variant (Y444F)-Cre · AAV2 variant (Y272F, Y444F, Y500F, Y730F)-Cre · AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F)-Cre · AAV2 variant(AAV2.7m8)-Cre · AAV5-Cre · AAV6-Cre · AAV8-Cre · AAV8-1m-Cre · AAV8-2m-Cre · AAV8 variant (Y733F, Y447F, Y275)-Cre · AAV9-Cre · AAV-Rh.10-Cre · AAV-DJ-Cre · AAV-DJ/8-Cre · AAV-Retro (Retrograde)-Cre · AAV9-PHP.B-Cre · AAV9-PHP.A-Cre · AAV9-PHP.eB-Cre · AAV9-PHP.S-Cre
c-fos
Neuro
Excitatory neuron promoter
AAV9-mecp2-Cre
AAV GeneMedi offer multiple serotypes of AAV-Cre loxP system for your option: · AAV1-Cre · AAV2-Cre · AAV2 variant (Y444F)-Cre · AAV2 variant (Y272F, Y444F, Y500F, Y730F)-Cre · AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F)-Cre · AAV2 variant(AAV2.7m8)-Cre · AAV5-Cre · AAV6-Cre · AAV8-Cre · AAV8-1m-Cre · AAV8-2m-Cre · AAV8 variant (Y733F, Y447F, Y275)-Cre · AAV9-Cre · AAV-Rh.10-Cre · AAV-DJ-Cre · AAV-DJ/8-Cre · AAV-Retro (Retrograde)-Cre · AAV9-PHP.B-Cre · AAV9-PHP.A-Cre · AAV9-PHP.eB-Cre · AAV9-PHP.S-Cre
mecp2
Neuro
Shorter neuron-specific promoter
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Abstract

Cre-loxP system is widely used in the field of biosciences, especially in the generation of genetically engineered mice (knockout or overexpression), enabling researchers to study the function of gene of interest (GOI). To date, numerous systems, derived from the basic Cre-loxP system, have been developed to precisely control gene expression at desired time or cells. Here, we briefly introduce Cre-loxP system, and give a summary about its applications, especially viral vectors-mediated Cre expression (such as AAV-Cre or Ad-Cre) for in vitro or in vivo studies.

AAV-DIO in neuroscience

In the research field of neuroscience, AAV-DIO shows outstanding spatiotemporal fidelity and is perfectly suitable for examining and manipulating neuronal function in vivo, such as studies in neural circuits and networks, behavior and so on. Optogenetics integrates optics with genetic engineering to measure or manipulate the biomolecular processes, especially in neuroscience [108]. By delivering optical signals with specific wavelength to precisely detect, measure, or control biological processes in living tissues, optogenetics opens up new landscapes for the study of biology, both in health and disease [109]. With the combination of AAV-DIO and optogenetics, researchers control and monitor the activities of individual neurons, and precisely measure the manipulation effects in real-time. For example, in Fig. 8, with AAV-DIO system, the neuron X specifically express opsin (such ChR2) and is labeled by the reporter gene (such as mCherry), but remains silenced. When exposed to light in correct wavelength, neuron X will be activated (Fig. 8) [110].

Figure 8. Principle of optogenetics tool in combination with AAV-DIO. With no light in specific wavelength matched to opsin, such as ChR2, ChR2 gene and the reporter gene is specifically expressed in neurons (location in red). Once exposed to light with the matched wavelength, ChR2 cation channels will be open and Na+ flow into neural cells. Thus, the neuron will be activated.

Genemedi possesses a complete library of optogenetics components into serotypes of AAV vector, which is listed in table 5. You can find more information on this website: https://www.genemedi.com/i/aav-optogenetics.

Table 5. List of optogenetics tools in AAV
AAV Plasmids Promoter and Expression Characteristics Fluorescent Protein Label Cre dependent Optogenetics effect
pAAV-CAG-DIO-eNpHR3.0-EYFPCAG general and strong expressionEYFPyesInhibition
pAAV-CAG -DIO-hChR2(H134R)-mCherry mCherryyesactivation
pAAV-CAG-DIO-ArchT-EYFP EYFPyesinhibition
pAAV-CAG -DIO-C1V1 (t/t)-TS-mCherry mCherryyesactivation
pAAV-CAG-DIO-Arch3.0-EYFP EYFPyesinhibition
pAAV- CAG -DIO-hCHETA-EYFP EYFPyesactivation
pAAV-CMV-DIO-eNpHR3.0-EYFPCMV(general and strong expression)EYFPyesinhibition
pAAV-CMV-DIO-hChR2(H134R)-mCherry mCherryyesactivation
pAAV-CMV-DIO-ArchT-EYFP EYFPyesinhibition
pAAV-CMV-DIO-C1V1 (t/t)-TS-mCherry mCherryyesactivation
pAAV-CMV-DIO-Arch3.0-EYFP EYFPyesinhibition
pAAV-CMV-DIO-hCHETA-EYFP EYFPyesactivation
pAAV-hSyn-eNpHR3.0-EYFPhSyn neuron specific expressionEYFPNoinhibition
pAAV-hSyn-hChR2(H134R)-mCherry mCherryNoactivation
pAAV-hSyn-ArchT-EYFP EYFPNoinhibition
pAAV-hSyn-C1V1-(t/t)-TS-mCherry mCherryNoactivation
pAAV-hSyn-Arch3.0-EYFP EYFPNoinhibition
pAAV-hSyn-DIO-hCHETA-EYFP EYFPyesactivation
pAAV-GFAP-eNpHR3.0-EYFPGFAP astrocyte specific expressionEYFPNoinhibition
pAAV-GFAP-hChR2(H134R)-mCherry mCherryNoactivation
pAAV-GFAP-ArchT-EYFP EYFPNoinhibition
pAAV-GFAP-C1V1 (t/t)-TS-mCherry mCherryNoactivation
pAAV-GFAP-Arch3.0-EYFP EYFPNoinhibition
pAAV-GFAP-hCHETA-EYFP EYFPNoactivation
pAAV-CaMKII-eNpHR3.0-EYFPCaMKII neuron specific expressionEYFPNoinhibition
pAAV-CaMKII-hChR2(H134R)-mCherry mCherryNoactivation
pAAV-CaMKII-ArchT-EYFP EYFPNoinhibition
pAAV-CaMKII-C1V1 (t/t)-TS-mCherry mCherryNoactivation
pAAV-CaMKII-Arch3.0-EYFP EYFPNoinhibition
pAAV-CaMKII-hCHETA-EYFP EYFPNoactivation

Summary

The Cre-loxP system, especially inducible tissue-specific knockout and viral vector-mediated Cre expression, has been highly utilized in genetics and cell biology research. Nevertheless, it seems that Cre-loxP system will continue to be prevalent in current and future research studies. GeneMedi is proficient in viral vector development and offers kinds of viral vector-based Cre tools, we can provide the best services and products if required.

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