Products & Information Collection of SARS-CoV-2 (2019nCOV) spike-HV 69-70 Deletion mutation(ΔH69/ΔV70)

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GeneMedi pseudotype virus (pseudovirus) of SARS-COV-2 (2019nCOV) spike-HV 69-70 Deletion mutation(ΔH69/ΔV70)

Taking responsibility to help accelerate the COVID-19 vaccine and therapeutic antibody discovery and development, GeneMedi had developed the pseudotype virus (pseudovirus) of SARS-COV-2 (2019nCOV) spike-HV 69-70 Deletion mutation(ΔH69/ΔV70), which will meet the evaluation of the efficacy of COVID19 vaccines and therapeutic antibodies.

GeneMedi codon-optimized spike mammalian expression vector for SARS-COV-2 (2019nCOV) spike-HV 69-70 Deletion mutation(ΔH69/ΔV70)

GeneMedi designed a mammalian expression codon-optimized spike mutation/deletion variant vector for COVID-19 SARS-COV-2 (2019nCOV) spike-HV 69-70 Deletion mutation (ΔH69/ΔV70).

SARS-CoV-2 spike-HV 69-70 Deletion mutation(ΔH69/ΔV70)

The world is in midst of the COVID-19 pandemic caused by SARS-CoV-2 (2019nCoV) infection. The Spike protein (S-protein) of SARS-CoV-2 (2019nCoV) mediates receptor (ACE2) binding and cell entry and is the dominant target of the immune system. Most mutations and deletions of SARS-CoV-2 occur in the coronavirus spike protein.

A deletion H69/V70 in Spike was present in over 3,000 sequences worldwide (2.5% of the available data), and largely in Europe from where most of the sequences in GISAID are derived.

The structural impact of the double deletion was predicted by homology modelling of the spike NTD possessing H69/V70 using SWISS-MODEL. The H69/V70 deletion was predicted to alter the conformation of a protruding loop comprising residues 69-76, with the loop being predicted to be predicted to be pulled in the NTD.

Recently a novel SARS-COV-2 (2019nCOV) lineage, the B.1.1.7 lineage, with serials of site mutation, shows stronger infection ability in the UK. In SARS-COV-2 B.1.1.7 lineage, most mutations and deletions occur in the coronavirus spike protein. These deletions also include SARS-CoV-2 spike-HV 69-70 Deletion mutation(ΔH69/ΔV70).

The mutations and deletions information of GeneMedi’s codon-optimized Spike vector for SARS-COV-2 B.1.1.7 lineage in UK COVID-19 pandemic recently:

21765-21770 deletion
21991-21993 deletion
A23063T
C23271A
C23604A
C23709T
T24506G
G24914C

Spike-S1 Subunit
Spike-S1 Subunit
Spike-S1 Subunit-RBD
Spike-S1 Subunit
Spike-S1 Subunit
Spike-S2 Subunit
Spike-S2 Subunit
Spike-S2 Subunit

HV 69-70 deletion (Click to more details about HV 69-70 deletion related products)
Y144 deletion
N501Y (Click to more details about N501Y related products)
A570D
P681H
T716I
S982A
D1118H

Gene Nucleotide Amino acid
ORF1abC3267TT1001I
C5388AA1708D
T6954CI2230T
11288-11296 deletionSGF 3675-3677 deletion
spike21765-21770 deletionHV 69-70 deletion (Click to more details
about HV 69-70 deletion related products)
21991-21993 deletionY144 deletion
A23063TN501Y (Click to more details
about N501Y related products)
C23271AA570D
C23604AP681H
C23709TT716I
T24506GS982A
G24914CD1118H
Orf8C27972TQ27stop
G28048TR52I
A28111GY73C
N28280 GAT->CTAD3L
C28977TS235F